Nkx-2.3 Antibody (4F4): sc-517182
- Nkx-2.3 Antibody (4F4) is a mouse monoclonal IgG2a κ Nkx-2.3 antibody provided at 100 µg/ml
- raised against amino acids 1-56 representing partial length Nkx-2.3 of human origin
- recommended for detection of Nkx-2.3 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nkx-2.3 Antibody (4F4). This work is in progress.
Nkx-2.3 Antibody (4F4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nkx-2.3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Nkx-2.3 antibody (4F4) is available as a non-conjugated format. Nkx-2.3 plays a crucial role in heart development, particularly during early stages of cardiac formation, where Nkx-2.3 acts as a transcriptional activator. This function helps regulate gene expression necessary for proper differentiation and proliferation of myocardial cells. Nkx-2.3 is primarily expressed in differentiated myocardial cells and is also found in gut mesoderm, brachial arches, tongue epithelium, and developing jaws. Nkx-2.3 contains a homeobox and a conserved NK2 domain, which are essential for transcriptional regulation. Abnormal expression levels of Nkx-2.3 can lead to significant cardiac anomalies, such as an enlarged heart due to increased myocardial thickness resulting from a higher number of myocardial cells. This underscores Nkx-2.3′s importance in maintaining normal cardiac structure and function, making anti-Nkx-2.3 antibody (4F4) a valuable tool for researchers studying heart development and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nkx-2.3 Antibody (4F4) References:
- Targeted disruption of the homeobox transcription factor Nkx2-3 in mice results in postnatal lethality and abnormal development of small intestine and spleen. | Pabst, O., et al. 1999. Development. 126: 2215-25. PMID: 10207146
- NKX2.3 is required for MAdCAM-1 expression and homing of lymphocytes in spleen and mucosa-associated lymphoid tissue. | Pabst, O., et al. 2000. EMBO J. 19: 2015-23. PMID: 10790368
- Transcriptional regulation of a mouse Clara cell-specific protein (mCC10) gene by the NKx transcription factor family members thyroid transciption factor 1 and cardiac muscle-specific homeobox protein (CSX). | Ray, MK., et al. 1996. Mol Cell Biol. 16: 2056-64. PMID: 8628271
- Chick NKx-2.3 represents a novel family member of vertebrate homologues to the Drosophila homeobox gene tinman: differential expression of cNKx-2.3 and cNKx-2.5 during heart and gut development. | Buchberger, A., et al. 1996. Mech Dev. 56: 151-63. PMID: 8798155
- NK-2 homeobox genes and heart development. | Harvey, RP. 1996. Dev Biol. 178: 203-16. PMID: 8812123
- Overexpression of the tinman-related genes XNkx-2.5 and XNkx-2.3 in Xenopus embryos results in myocardial hyperplasia. | Cleaver, OB., et al. 1996. Development. 122: 3549-56. PMID: 8951070
- Vertebrate tinman homologues XNkx2-3 and XNkx2-5 are required for heart formation in a functionally redundant manner. | Fu, Y., et al. 1998. Development. 125: 4439-49. PMID: 9778503
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nkx-2.3 Antibody (4F4) | sc-517182 | 100 µg/ml | $316.00 |