Date published: 2026-4-24

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Nitrofluorescein, Isomer 1 (CAS 3326-35-0)

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Alternate Names:
5-Nitrofluorescein
Application:
Nitrofluorescein, Isomer 1 is a fluorescent labeling reagent for proteins
CAS Number:
3326-35-0
Molecular Weight:
377.30
Molecular Formula:
C20H11NO7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Nitrofluorescein, Isomer 1 is a fluorescent labeling reagent for proteins which is used in the fluorescent antibody technique for rapid identification of pathogens.


Nitrofluorescein, Isomer 1 (CAS 3326-35-0) References

  1. Isothiocyanate compounds as fluorescent labeling agents for immune serum.  |  RIGGS, JL., et al. 1958. Am J Pathol. 34: 1081-97. PMID: 13583098
  2. FLUORESCENT ANTIBODY METHODS AND THEIR USE IN MALARIA RESEARCH.  |  VOLLER, A. 1964. Bull World Health Organ. 30: 343-54. PMID: 14163958
  3. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.  |  COONS, AH. and KAPLAN, MH. 1950. J Exp Med. 91: 1-13. PMID: 15395569
  4. Direct detection of the oxygen rebound intermediates, ferryl Mb and NO2, in the reaction of metmyoglobin with peroxynitrite.  |  Su, J. and Groves, JT. 2009. J Am Chem Soc. 131: 12979-88. PMID: 19705829
  5. The fluorescent antibody method in medical and biological research.  |  Borek, F. 1961. Bull World Health Organ. 24: 249-56. PMID: 20604086
  6. Mechanisms of peroxynitrite interactions with heme proteins.  |  Su, J. and Groves, JT. 2010. Inorg Chem. 49: 6317-29. PMID: 20666389
  7. Rotational and vibrational dynamics in the excited electronic state of deprotonated and protonated fluorescein studied by time-resolved photofragmentation in an ion trap.  |  Imanbaew, D., et al. 2016. Struct Dyn. 3: 043211. PMID: 27376104
  8. Assay of materials used in immunofluorescence techniques.  |  Brighton, WD. 1966. J Clin Pathol. 19: 456-60. PMID: 4162128
  9. Protein polypeptide chain molecular weights by gel chromatography in guanidinium chloride.  |  Mann, KG. and Fish, WW. 1972. Methods Enzymol. 26: 28-42. PMID: 4680709

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Nitrofluorescein, Isomer 1, 1 g

sc-212398
1 g
$300.00