NIT1 Antibody (D-7): sc-515566
- NIT1 Antibody (D-7) is a mouse monoclonal IgG1 κ NIT1 antibody provided at 200 µg/ml
- raised against amino acids 78-205 mapping within an internal region of NIT1 of human origin
- NIT1 Antibody (D-7) is recommended for detection of NIT1 of human origin by WB, IP, IF and ELISA
- Anti-NIT1 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NIT1 Antibody (D-7) for WB applications. These reagents are now offered in bundles with NIT1 Antibody (D-7) (see ordering information below).
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NIT1 Antibody (D-7) is a mouse monoclonal IgG1 antibody that detects NIT1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NIT1 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NIT1, or nitrilase homolog 1, is a 327 amino acid protein that plays a crucial role in regulating cell growth and apoptosis, functioning as a tumor suppressor. NIT1 is primarily localized in the cytoplasm and mitochondria of various tissues, including the placenta, kidney, brain, liver, heart, pancreas, and skeletal muscle, which is significant as NIT1 presence in these areas is essential for maintaining cellular homeostasis and responding to stress signals. Notably, NIT1 is involved in the metabolism of nitriles and organic amides, cleaving them to produce carboxylic acids and ammonia, which are vital for various metabolic processes. NIT1 expression levels are tightly regulated; loss of NIT1 expression can lead to accelerated cell proliferation and increased resistance to DNA damage, while NIT1 overexpression can trigger caspase-dependent apoptosis. Additionally, NIT1 exists in four isoforms due to alternative splicing, which may contribute to NIT1 functional diversity and regulatory mechanisms in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NIT1 Antibody (D-7) References:
- Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster. | Vorwerk, S., et al. 2001. Planta. 212: 508-16. PMID: 11525507
- The nitrilase family of CN hydrolysing enzymes - a comparative study. | O'Reilly, C. and Turner, PD. 2003. J Appl Microbiol. 95: 1161-74. PMID: 14632988
- Imaging plant cell death: GFP-Nit1 aggregation marks an early step of wound and herbicide induced cell death. | Cutler, SR. and Somerville, CR. 2005. BMC Plant Biol. 5: 4. PMID: 15796778
- Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor. | Semba, S., et al. 2006. J Biol Chem. 281: 28244-53. PMID: 16864578
- Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes. | Barglow, KT., et al. 2008. Biochemistry. 47: 13514-23. PMID: 19053248
- Mammalian nitrilase 1 homologue Nit1 is a negative regulator in T cells. | Zhang, H., et al. 2009. Int Immunol. 21: 691-703. PMID: 19395373
- Nit1 and Fhit tumor suppressor activities are additive. | Sun, J., et al. 2009. J Cell Biochem. 107: 1097-106. PMID: 19479888
- Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans. | Pekarsky, Y., et al. 1998. Proc Natl Acad Sci U S A. 95: 8744-9. PMID: 9671749
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NIT1 Antibody (D-7) | sc-515566 | 200 µg/ml | $322.00 | |||
NIT1 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-531419 | 200 µg Ab; 10 µg BP | $361.00 | |||
NIT1 Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-525105 | 200 µg Ab, 40 µg BP | $361.00 | |||
NIT1 Antibody (D-7): m-IgG1 BP-HRP Bundle | sc-544543 | 200 µg Ab; 20 µg BP | $361.00 | |||
NIT1 Antibody (D-7) AC | sc-515566 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NIT1 Antibody (D-7) HRP | sc-515566 HRP | 200 µg/ml | $322.00 | |||
NIT1 Antibody (D-7) FITC | sc-515566 FITC | 200 µg/ml | $336.00 | |||
NIT1 Antibody (D-7) PE | sc-515566 PE | 200 µg/ml | $349.00 | |||
NIT1 Antibody (D-7) Alexa Fluor® 488 | sc-515566 AF488 | 200 µg/ml | $364.00 | |||
NIT1 Antibody (D-7) Alexa Fluor® 546 | sc-515566 AF546 | 200 µg/ml | $364.00 | |||
NIT1 Antibody (D-7) Alexa Fluor® 594 | sc-515566 AF594 | 200 µg/ml | $364.00 | |||
NIT1 Antibody (D-7) Alexa Fluor® 647 | sc-515566 AF647 | 200 µg/ml | $364.00 | |||
NIT1 Antibody (D-7) Alexa Fluor® 680 | sc-515566 AF680 | 200 µg/ml | $364.00 | |||
NIT1 Antibody (D-7) Alexa Fluor® 790 | sc-515566 AF790 | 200 µg/ml | $364.00 |