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Ninhydrin reagent, 2% solution (CAS 485-47-2 (solid)) - chemical structure image
Molecular structure of Ninhydrin reagent, 2% solution, CAS Number: 485-47-2 (solid)
Ninhydrin reagent, 2% solution (CAS 485-47-2 (solid)) - chemical structure image
Molecular structure of Ninhydrin reagent, 2% solution, CAS Number: 485-47-2 (solid)
Molecular structure of Ninhydrin reagent, 2% solution, CAS Number: 485-47-2 (solid)

Ninhydrin reagent, 2% solution in DMSO

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Application:
Ninhydrin reagent, 2% solution is a useful reagent for detecting amines
Molecular Weight:
178.14
Molecular Formula:
C9H6O4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Description
Technical Information
Safety Information
SDS & Certificate of Analysis

Ninhydrin reagent, 2% solution, is an effective reagent for thin-layer chromatography (TLC). Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product (diketohydrindylidene-diketohydrindamine). Ninhydrin is used for the development of fingermarks on porous surfaces and is often used in comparison with other latent fingerprint techniques. Studies have indicated that Ninhydrin may be useful for detecting ninhydrin-reactive nitrogens in soil for forensic applications. Other studies have indicated that the color developed after reaction of ninhydrin and amino acids can vary depending on the composition and type of amino acids. Additionally, ninhydrin has been noted to be useful in the analysis of free amino groups in proteins, peptides and amino acids.


Ninhydrin reagent, 2% solution in DMSO References

  1. Using ninhydrin to detect gravesoil.  |  Carter, DO., et al. 2008. J Forensic Sci. 53: 397-400. PMID: 18366573
  2. Modelling the simultaneous processes occurring during the BOD(5) test using synthetic waters as a pattern.  |  Martín, MJ., et al. 2009. Bioprocess Biosyst Eng. 32: 207-16. PMID: 18546022
  3. Thermal development of latent fingermarks on porous surfaces--further observations and refinements.  |  Song, DF., et al. 2011. Forensic Sci Int. 204: 97-110. PMID: 20554406
  4. The release characteristics of a model protein from self-assembled succinimide-terminated poly(lactide-co-glycolide ethylene oxide fumarate) nanoparticles.  |  Mercado, AE., et al. 2008. Nanotechnology. 19: 325609. PMID: 21828822
  5. Preparative-scale kinetic resolution of racemic styrene oxide by immobilized epoxide hydrolase.  |  Yildirim, D., et al. 2011. Enzyme Microb Technol. 49: 555-9. PMID: 22142731
  6. A mechanistic analysis of the quantitation of α-hydroxy ketones by the bicinchoninic acid assay.  |  Weiser, JR., et al. 2012. Anal Biochem. 430: 116-22. PMID: 22910661
  7. Brain-targeted delivery of protein using chitosan- and RVG peptide-conjugated, pluronic-based nano-carrier.  |  Kim, JY., et al. 2013. Biomaterials. 34: 1170-8. PMID: 23122677
  8. A novel concentration dependent amino acid ion pair strategy to mediate drug permeation using indomethacin as a model insoluble drug.  |  ElShaer, A., et al. 2014. Eur J Pharm Sci. 62: 124-31. PMID: 24907680
  9. Synthesis of L-lysine imprinted cryogels for immunoglobulin G adsorption.  |  Çulha, S., et al. 2015. Mater Sci Eng C Mater Biol Appl. 52: 315-24. PMID: 25953573
  10. Characterization of polymeric nanoparticles for intravenous delivery: Focus on stability.  |  Oliveira, CL., et al. 2017. Colloids Surf B Biointerfaces. 150: 326-333. PMID: 27823851
  11. A novel serum-stable liver targeted cytotoxic system using valerate-conjugated chitosan nanoparticles surface decorated with glycyrrhizin.  |  El-Marakby, EM., et al. 2017. Int J Pharm. 525: 123-138. PMID: 28392279
  12. Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes.  |  Lakshmanan, V., et al. 2017. Methods Mol Biol. 1631: 349-362. PMID: 28735410
  13. Acids 'generally recognized as safe' affect morphology and biocompatibility of electrospun chitosan/polyethylene oxide nanofibers.  |  Stie, MB., et al. 2019. Carbohydr Polym. 215: 253-262. PMID: 30981352
  14. A Novel Chitosan Nanosponge as a Vehicle for Transepidermal Drug Delivery.  |  Lee, JS., et al. 2021. Pharmaceutics. 13: PMID: 34575405
  15. Reduced cellulose accessibility slows down enzyme-mediated hydrolysis of cellulose.  |  Wu, J., et al. 2023. Bioresour Technol. 371: 128647. PMID: 36681353
  16. Third supplement for the paper chromatographic separation and identification of phenol derivatives and related compounds of biochemical interest using a 'reference system'.  |  Reio, L. 1970. J Chromatogr. 47: 60-85. PMID: 5418121
  17. Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction.  |  Moore, S. 1968. J Biol Chem. 243: 6281-3. PMID: 5723468
  18. A specific quantitative colorimetric assay for L-asparagine.  |  Sheng, S., et al. 1993. Anal Biochem. 211: 242-9. PMID: 8100404

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ninhydrin reagent, 2% solution in DMSO, 100 ml

sc-360255
100 ml
$75.00

I have lot of F0917 provide CoA with an expiration date

Asked by: sandip
Thank you for your question. CoA documentation can be downloaded directly from the corresponding product page. Just enter the lot number and, if required, your contact information.
Answered by: Tech Support Europe
Date published: 2022-11-16

Can this reagent be used as a TLC spray reagent directly or is this product not suitable for TLC purposes?

Asked by: zoela
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-15

hello, i have a batch of reagent (H2918), the COA dose not have an expiration date\retest date. can you send me a COA with the expiration? I tried to contact your technical service from 08/11, and I have no answer, can you help me please

Asked by: ruba123
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2020-12-09

What is the relative % amount of DMSO and lithium acetate buffer in which the solution is prepared?

Asked by: fcaiazza
Please see section 3 of our SDS for the components in this solution.
Answered by: Technical Support
Date published: 2019-05-03

hello, i have a batch of reagent (D1317), the COA dose not have an expiration date\retest date. can you send me a COA with the expiration?

Asked by: enzylab
Thank you for your question. Please contact our technical service so we can send you an updated CoA for this lot of sc-360255 with the expiry date.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-07

Is 20microliter of ninhydrin can detect 20microliter of 0.1mM glycine.

Asked by: rashi
Thank you for your question. Stock solutions of amino acids can be made at a concentration of 0.05umoles/ml (50uM) in 0.05% glacial acetic acid.
Answered by: Technical Support
Date published: 2020-12-02

Needs Quotation

Asked by: anilrai
Thank you for your question. Please contact one of our authorized distributors in India to request an accurate quotation for this chemical. A link to search for our distributors on our website is provided below. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-03-21

please tell me How to prepare 2% ninhydrin solution.

Asked by: manmadhareddi
Thank you for your question. Our product, Ninhydrin reagent, 2% solution: sc-360255, is provided as a 2% solution in DMSO and lithium acetate buffer, pH 5.2.
Answered by: Technical Support
Date published: 2021-11-20
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Rated 5 out of 5 by from Various publications cite the use of NinhydrinVarious publications cite the use of Ninhydrin reagent, 2% solution for thin-layer chromatography. Ninhydrin is commonly used for the development of fingermarks on porous surfaces and is often used in comparison with other latent fingerprint techniques. Studies have indicated that Ninhydrin may be useful for detecting ninhydrin-reactive nitrogens in soil for forensic applications. -SCBT Publication Review
Date published: 2015-07-10
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Ninhydrin reagent, 2% solution in DMSO is rated 5.0 out of 5 by 1.
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