Date published: 2026-4-5

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Ninhydrin (CAS 485-47-2)

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Application:
Ninhydrin is a useful reagent for detecting amines
CAS Number:
485-47-2
Purity:
≥99%
Molecular Weight:
178.14
Molecular Formula:
C9H6O4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Ninhydrin is used to detect the presence of amino acids and proteins in biological samples. When in contact with these compounds, ninhydrin forms a purple or blue color, allowing for easy visualization and quantification of the amino acids or proteins present. This reaction is used to analyze and identify biological samples. Ninhydrin is particularly useful in chromatography and fingerprint analysis, where it helps to reveal and distinguish different compounds based on their amino acid content. Its ability to selectively react with amino acids makes it useful in various biochemical and analytical applications.


Ninhydrin (CAS 485-47-2) References

  1. Using ninhydrin to detect gravesoil.  |  Carter, DO., et al. 2008. J Forensic Sci. 53: 397-400. PMID: 18366573
  2. Thermal development of latent fingermarks on porous surfaces--further observations and refinements.  |  Song, DF., et al. 2011. Forensic Sci Int. 204: 97-110. PMID: 20554406
  3. Ninhydrin reaction on thiol-reactive solid and its potential for the quantitation of D-penicillamine.  |  Rojanarata, T., et al. 2010. Talanta. 82: 444-9. PMID: 20602918
  4. Critical evaluation of ninhydrin for monitoring surgical instrument decontamination.  |  Nayuni, NK., et al. 2013. J Hosp Infect. 84: 97-102. PMID: 23518458
  5. New Horizons for Ninhydrin: Colorimetric Determination of Gender from Fingerprints.  |  Brunelle, E., et al. 2016. Anal Chem. 88: 2413-20. PMID: 26753919
  6. Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins.  |  Anantharaman, S., et al. 2017. Spectrochim Acta A Mol Biomol Spectrosc. 173: 897-903. PMID: 27821372
  7. A synthesis of oxo-thioxo[3.3.3]propellanes from dithiocarbamates and ninhydrin-malononitrile adduct.  |  Yavari, I. and Khajeh-Khezri, A. 2017. Mol Divers. 21: 849-854. PMID: 28653127
  8. Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma.  |  Omar, MA., et al. 2018. Luminescence. 33: 1107-1112. PMID: 29968975
  9. A Ninhydrin-Type Urea Sorbent for the Development of a Wearable Artificial Kidney.  |  Jong, JAW., et al. 2020. Macromol Biosci. 20: e1900396. PMID: 32065727
  10. Applicability of ninhydrin as a fluorescent reagent for estimation of teicoplanin in human plasma using salting-out assisted liquid-liquid extraction technique.  |  Ali, MFB., et al. 2020. Luminescence. 35: 1118-1124. PMID: 32436350
  11. Ninhydrin as a novel DNA hybridization indicator applied to a highly reusable electrochemical genosensor for Candida auris.  |  Guedes, PHG., et al. 2021. Talanta. 235: 122694. PMID: 34517578
  12. Organocatalytic Regio- and Enantioselective [3 + 2]-Annulations of Ninhydrin-Derived Morita-Baylis-Hillman Carbonates with 3-Methyleneoxindoles.  |  Lu, Z., et al. 2022. J Org Chem. 87: 3184-3194. PMID: 35133821
  13. A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent.  |  Haeger, G., et al. 2022. Anal Biochem. 654: 114819. PMID: 35839914
  14. Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction.  |  Moore, S. 1968. J Biol Chem. 243: 6281-3. PMID: 5723468
  15. A specific quantitative colorimetric assay for L-asparagine.  |  Sheng, S., et al. 1993. Anal Biochem. 211: 242-9. PMID: 8100404

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ninhydrin, 10 g

sc-203162
10 g
$44.00

Ninhydrin, 25 g

sc-203162A
25 g
$64.00

Ninhydrin, 100 g

sc-203162B
100 g
$189.00