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Nigrosin (CAS 8005-03-6)

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Alternate Names:
Acid Black 2
Application:
Nigrosin is a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi
CAS Number:
8005-03-6
Molecular Weight:
616.49
Molecular Formula:
C22H14N6Na2O9S2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Nigrosin is used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi. Negative staining with nigrosin can reveal some microorganisms that cannot be stained by regular methods.


Nigrosin (CAS 8005-03-6) References

  1. Analysis and testing of biological stains--the Biological Stain Commission Procedures.  |  Penney, DP., et al. 2002. Biotech Histochem. 77: 237-75. PMID: 12564600
  2. Nigrosin as a dye for differentiating live and dead ascites cells.  |  KALTENBACH, JP., et al. 1958. Exp Cell Res. 15: 112-7. PMID: 13574164
  3. [Nigrosin staining of the cerebral cortex].  |  VONWILLER, P. and HUFSCHMIDT, H. 1950. Monatsschr Psychiatr Neurol. 120: 411-8. PMID: 14806306
  4. Negative-staining of protozoa with nigrosin.  |  ROSENBAUM, RM. 1949. Stain Technol. 24: 79-84. PMID: 18116080
  5. Decolorization of naphthol blue black using the horseradish peroxidase.  |  Onder, S., et al. 2011. Appl Biochem Biotechnol. 163: 433-43. PMID: 20703828
  6. Sonochemical degradation of naphthol blue black in water: Effect of operating parameters.  |  Ferkous, H., et al. 2015. Ultrason Sonochem. 26: 40-47. PMID: 25843901
  7. Naphthol Blue Black and 99mTc-Labeled Mannosylated Human Serum Albumin (99mTc-MSA) Conjugate as a Multimodal Lymph Node Mapping Nanocarrier.  |  Lee, JY., et al. 2018. Sci Rep. 8: 13636. PMID: 30206287
  8. Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling.  |  Sari, LM., et al. 2020. Front Vet Sci. 7: 610597. PMID: 33479599
  9. Negative staining method with nigrosin for the detection of cryptosporidial oocysts: a comparative study.  |  Pohjola, S. 1984. Res Vet Sci. 36: 217-9. PMID: 6201968
  10. Elastin VII: aging effects on vascular elastica staining by oil soluble nigrosin dyes.  |  Lillie, RD., et al. 1976. Virchows Arch A Pathol Anat Histol. 371: 323-30. PMID: 62442

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Nigrosin, 25 g

sc-215566
25 g
$34.00

Nigrosin, 100 g

sc-215566A
100 g
$107.00