NIF3L1 BP1 Double Nickase Plasmid (m): sc-425902-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NIF3L1 BP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NIF3L1 BP1 Double Nickase Plasmid (m) and NIF3L1 BP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Thoc7. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NIF3L1 BP1 Double Nickase Plasmid (m) | sc-425902-NIC | 20 µg | $410.00 | |||
NIF3L1 BP1 Double Nickase Plasmid (m2) | sc-425902-NIC-2 | 20 µg | $410.00 |
Mouse Thoc7 encodes a component of the THO/TREX ribonucleoprotein complex that supports co‑transcriptional mRNA processing and efficient export of mature transcripts from the nucleus. Through coupling transcription elongation with mRNP assembly, THOC7 helps maintain RNA quality control and genome stability, influencing cellular programs such as proliferation and differentiation. Disruption of TREX-associated factors has been linked to aberrant RNA metabolism, transcription–replication conflicts, and stress responses that are frequently implicated in cancer biology and neurodevelopmental phenotypes. As a result, Thoc7 is a useful target for dissecting how RNA export and R-loop homeostasis shape gene-expression networks in mammalian cells.
NIF3L1 BP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Thoc7 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Thoc7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Thoc7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Thoc7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.