Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nidogen Double Nickase Plasmid (m): sc-421895-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nidogen Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nidogen Double Nickase Plasmid (m) and Nidogen Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nid1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nidogen Antibody (C-7): sc-133175
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nidogen Double Nickase Plasmid (m) | sc-421895-NIC | 20 µg | $410.00 | |||
Nidogen Double Nickase Plasmid (m2) | sc-421895-NIC-2 | 20 µg | $410.00 |
Mouse Nid1 encodes nidogen (entactin), a sulfated basement membrane glycoprotein that bridges laminin and type IV collagen networks to stabilize extracellular matrix architecture. Nidogen supports cell–matrix adhesion, migration, and mechanotransduction by organizing basement membrane protein assemblies and influencing integrin-associated signaling and tissue morphogenesis. Nid1-dependent matrix integrity is relevant to studies of vascular and epithelial barrier function, neuromuscular junction organization, and organ development where basement membrane defects can alter differentiation and tissue homeostasis. Dysregulated nidogen-containing matrices are frequently examined in fibrosis, tumor microenvironment remodeling, and inflammatory injury models as determinants of permissive or restrictive tissue niches.
Nidogen Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nid1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nid1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nid1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nid1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.