Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Double Nickase Plasmid (h): sc-416614-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Double Nickase Plasmid (h) and Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHRNA7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319): sc-58607
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Double Nickase Plasmid (h) | sc-416614-NIC | 20 µg | $410.00 | |||
Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Double Nickase Plasmid (h2) | sc-416614-NIC-2 | 20 µg | $410.00 |
CHRNA7 encodes the α7 subunit of the nicotinic acetylcholine receptor, a homopentameric ligand-gated cation channel that mediates rapid cholinergic signaling and calcium influx. α7 nAChR activity influences intracellular Ca²⁺-dependent pathways including MAPK/ERK, PI3K–AKT, and CaMK signaling, thereby shaping neurotransmitter release, synaptic plasticity, and neuronal excitability. In immune and glial contexts, CHRNA7 contributes to modulation of inflammatory responses through cholinergic regulation of cytokine production. Dysregulated CHRNA7 expression or function has been linked to neuropsychiatric and neurodegenerative disease biology, and it is widely studied in the context of cognition, sensory gating, and inflammation-associated neural dysfunction.
Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHRNA7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHRNA7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHRNA7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHRNA7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.