RELA/NFκB p65 (m): 293T Lysate: sc-122027

NFκB p65 (m): 293T Lysate is derived from the human embryonic kidney 293T cell line, genetically engineered to express the mouse NFκB p65 subunit, a crucial component of the NFκB signaling pathway. NFκB p65, also known as RelA, plays a vital role in regulating immune and inflammatory responses, cell proliferation, and survival. The 293T cell line is ideal for this purpose due to its high transfection efficiency and robust protein expression. NFκB p65 functions by forming heterodimers with other NFκB family members, predominantly p50, and translocating to the nucleus upon activation, where it binds to specific DNA sequences to regulate target gene expression. In research, NFκB p65 (m): 293T Lysate is extensively used to study the mechanisms underlying NFκB activation and signaling. This lysate is instrumental in biochemical assays such as Western blotting, immunoprecipitation, and electrophoretic mobility shift assays (EMSAs) to analyze NFκB p65 expression, phosphorylation status, and DNA-binding activity. Additionally, it is used in reporter gene assays to investigate NFκB-dependent transcriptional activity. Researchers employ this lysate to explore the cross-talk between NFκB signaling and other cellular pathways, shedding light on its role in cellular responses to stress, cytokines, and other stimuli. This lysate advances our understanding of NFκB-mediated gene regulation and its broader implications in cellular physiology.