NFκB p52/p100/NFKB2 Double Nickase Plasmid (h): sc-400418-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFκB p52/p100/NFKB2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NFκB p52/p100/NFKB2 Double Nickase Plasmid (h) and NFκB p52/p100/NFKB2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NFKB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFκB p52/p100/NFKB2 Antibody (C-5): sc-7386
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFκB p52/p100/NFKB2 Double Nickase Plasmid (h) | sc-400418-NIC | 20 µg | $410.00 | |||
NFκB p52/p100/NFKB2 Double Nickase Plasmid (h2) | sc-400418-NIC-2 | 20 µg | $410.00 |
NFKB2 encodes the NFκB p100 precursor that is processed to the p52 subunit, a central transcriptional regulator in the noncanonical NF-κB pathway. Through regulated p100 processing downstream of receptors such as BAFFR, CD40, and LTβR, NFκB2 controls B-cell maturation, lymphoid organogenesis, cytokine signaling, and survival programs. Its activity integrates with IKKα–NIK signaling, RelB-containing dimers, and crosstalk with canonical NF-κB responses to shape inflammatory and immune gene expression. Dysregulated NFKB2 signaling has been associated with immune dysfunction and lymphoid malignancy biology, making it a frequent target for mechanistic studies of signaling, transcriptional control, and cell fate decisions.
NFκB p52/p100/NFKB2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NFKB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NFKB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NFKB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NFKB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.