NFATc1 Double Nickase Plasmid (m): sc-421863-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFATc1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NFATc1 Double Nickase Plasmid (m) and NFATc1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nfatc1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFATc1 Antibody (7A6): sc-7294
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFATc1 Double Nickase Plasmid (m) | sc-421863-NIC | 20 µg | $410.00 |
Nfatc1 encodes the transcription factor NFATc1, a calcium/calcineurin-regulated member of the NFAT family that integrates Ca²⁺ signaling with transcriptional programs controlling immune activation and tissue remodeling. In mouse cells, NFATc1 is rapidly induced downstream of T cell receptor signaling and cooperates with AP-1 to regulate cytokine genes, differentiation states, and activation-dependent transcriptional networks. Beyond lymphocytes, NFATc1 contributes to osteoclastogenesis and bone resorption through RANKL-driven pathways and intersects with MAPK and NF-κB signaling to shape lineage-specific gene expression. Dysregulated NFATc1 activity has been associated with inflammatory phenotypes and remodeling processes relevant to autoimmune-like pathology, bone loss mechanisms, and tumor microenvironment signaling in preclinical models.
NFATc1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nfatc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nfatc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nfatc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nfatc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.