NFATc1 Double Nickase Plasmid (h): sc-400164-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFATc1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NFATc1 Double Nickase Plasmid (h) and NFATc1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NFATC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFATc1 Antibody (7A6): sc-7294
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFATc1 Double Nickase Plasmid (h) | sc-400164-NIC | 20 µg | $410.00 | |||
NFATc1 Double Nickase Plasmid (h2) | sc-400164-NIC-2 | 20 µg | $410.00 |
NFATC1 encodes NFATc1, a calcium-regulated transcription factor activated downstream of calcineurin in response to T cell receptor signaling and other immunoreceptor pathways. Upon dephosphorylation, NFATc1 translocates to the nucleus to coordinate transcriptional programs that shape immune activation, cytokine expression, and differentiation states, and it also contributes to osteoclastogenesis and tissue remodeling programs. NFATc1 integrates Ca2+/NFAT signaling with MAPK- and AP-1–dependent inputs to fine-tune context-specific gene expression. Dysregulated NFATC1 activity has been implicated in immune and inflammatory phenotypes as well as cancer-associated transcriptional reprogramming, supporting its utility as a node for mechanistic pathway studies.
NFATc1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NFATC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NFATC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NFATC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NFATC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.