Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evalutaion of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm.
1. Antal, P., et al. 1995. Ann. Hematol. 70: 259-265. PMID: 7599287 2. Ricci, V., et al. 2002. Biochem. Biophys. Res. Commun. 292: 167-174. PMID: 11890688 3. Ni, Y., et al. 2006. Anal. Biochem. 352: 231-242. PMID: 16584704 4. Link, C.D., et al. 2006. J. Biol. Chem. 281: 1808-1816. PMID: 16239215
Soluble in water (50 mg/ml at 25° C), abs. alcohol (1.8%), and cellosolve (3.75%).
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Various publications cite the use of NeutralVarious publications cite the use of Neutral Red for histological staining of lysosymes, but it has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evaluation of synthetic and naturally derived biomaterials. -SCBT Publication Review
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