neuropilin Antibody (A-12)recommended for detection of neuropilin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

neuropilin Antibody (A-12): sc-5307

neuropilin Antibody (A-12) is rated 4.6 out of 5 by 11.
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Datasheets

    • neuropilin Antibody (A-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 570-855 of neuropilin of human origin
    • neuropilin Antibody (A-12) is recommended for detection of neuropilin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • neuropilin Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of neuropilin (A-12): sc-5307.
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How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-5307 HRP), phycoerythrin (sc-5307 PE), fluoroscein (sc-5307 FITC), Alexa Fluor® 488 (sc-5307 AF488) and Alexa Fluor® 647 (sc-5307 AF647).
Answered by: Tech Service
Date published: 2017-01-27
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Rated 5 out of 5 by from great results I received free samples and tried on western blotting. Tha bands are thick with no secondary bands. Need to dilute antibody concentrations to get good data.
Date published: 2018-02-12
Rated 5 out of 5 by from Anticorpo versatile ed altamente specifico Ho utilizzato questo anticorpo sia per western blot che per neutralizzazione della NRP1 in cellule di melanoma umano con ottimi risultati.
Date published: 2018-02-10
Rated 5 out of 5 by from PDGFC and neuropilin-1 in melanoma The antibody has allowed to demonstrate for the first time the capability of NRP-1 expressing melanoma cells to be stimulated by PDGFC
Date published: 2018-01-16
Rated 1 out of 5 by from Aucun signal sur des cellules exprimant NRP1 Lyse Laemmli ou bien RIPA de cellules 786-O ou HUVEC exprimant NRP1 et NRP2. Les mêmes extraits ont été déposés sur gel WB 9% puis la membrane hybridée avec différents anticorps contre NRP1 (différents fournisseurs). Seul le Santa Cruz ne donne pas de signal
Date published: 2017-11-30
Rated 5 out of 5 by from Highly recommended for IHC(P) and WB. This is a well characterized monoclonal antibody with tons of citations in the literature for a wide variety of applications. Great Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue, and Immunofluorescence staining of methanol-fixed HeLa cells.
Date published: 2016-12-23
Rated 5 out of 5 by from Published data for WB and IF in human kidney Published data for WB and IF in human kidney and PTC cells. -SCBT Publication Review
Date published: 2015-05-15
Rated 5 out of 5 by from Published IHC data for use in human breast Published IHC data for use in human breast cancer tissue. -SCBT Publication Review
Date published: 2015-05-08
Rated 5 out of 5 by from Published data for IP and WB in human HEK Published data for IP and WB in human HEK cells and porcine PAE cells. -SCBT Publication Review
Date published: 2015-04-12
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