Neurophysin I Double Nickase Plasmid (h): sc-410922-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neurophysin I Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Neurophysin I Double Nickase Plasmid (h) and Neurophysin I Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OXT. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neurophysin I Double Nickase Plasmid (h) | sc-410922-NIC | 20 µg | $410.00 | |||
Neurophysin I Double Nickase Plasmid (h2) | sc-410922-NIC-2 | 20 µg | $410.00 |
Human OXT encodes the oxytocin prepropeptide that is processed into the oxytocin nonapeptide and its carrier protein neurophysin I, which together support peptide maturation, stabilization, and regulated secretion from the neurohypophyseal system. Oxytocin signaling primarily engages the oxytocin receptor to activate Gq/PLCβ-dependent calcium mobilization and downstream MAPK pathways, influencing neuroendocrine release programs and tissue-specific contractile and secretory responses. In the nervous system, OXT-linked signaling contributes to circuit-level modulation of social and stress-related behaviors, while peripheral actions integrate with reproductive and metabolic physiology. Dysregulation of oxytocinergic pathways and peptide processing has been investigated in neurodevelopmental and psychiatric phenotypes, as well as disorders affecting hypothalamic–pituitary function, making OXT a relevant target for mechanistic studies of neuropeptide biology.
Neurophysin I Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OXT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OXT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OXT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OXT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.