Neuron navigator 2 CRISPR/Cas9 KO Plasmid (m): sc-429641
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neuron navigator 2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neuron navigator 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neuron navigator 2 CRISPR/Cas9 KO Plasmid (m) | sc-429641 | 20 µg | $397.00 |
Overview
Nav2 encodes neuron navigator 2, a neuron-enriched cytoskeletal-associated protein implicated in neurite extension, axon guidance, and neuronal migration through coordination of microtubule dynamics and growth cone behavior. In developing and mature mouse nervous system, NAV2 is linked to processes that shape neural circuit formation, including polarity establishment and directed outgrowth in response to guidance cues. Altered NAV2-associated pathways have been studied in the context of neurodevelopmental phenotypes and nervous system dysfunction where disrupted connectivity and axonal patterning are central features. As a result, Nav2 is a useful target for interrogating mechanisms of neuronal morphogenesis and cytoskeletal remodeling in disease-relevant cellular models.
Neuron navigator 2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nav2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nav2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nav2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neuron navigator 2 protein expression.
This CRISPR knockout system enables efficient generation of Nav2-deficient cell models for investigation of Neuron navigator 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nav2 exon(s) critical for Neuron navigator 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nav2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neuron navigator 2 CRISPR/Cas9 KO Plasmid (m) and Neuron navigator 2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nav2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neuron navigator 2 HDR Plasmid (m) and Neuron navigator 2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nav2 homology arms to support homology-directed repair at defined Nav2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.