Neurofibromin Antibody (H-12): sc-376886

Neurofibromin Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Neurofibromin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Neurofibromin antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neurofibromin, also known as NF1-GAP-related protein, plays a crucial role in regulating cell growth and differentiation by acting as a negative regulator of the RAS signaling pathway, which is vital for normal cellular function and development. Mutations in the NF1 gene can lead to neurofibromatosis type 1, a common autosomal dominant disorder characterized by the development of benign tumors and an increased risk of certain malignancies. Neurofibromin consists of 2,818 amino acids and is expressed consistently across various tissues, including brain, lung, liver, kidney, spleen, muscle, and colon, highlighting its importance in maintaining cellular homeostasis. Structural homology with the catalytic domain of GTPase-activating proteins suggests Neurofibromin may interact with Ras proteins in vivo, further emphasizing its critical role in cellular signaling pathways.

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Neurofibromin Antibody (H-12) References:

1. On the lysosomal degradation of neurofibromin and its phosphorylation in cultured melanocytes.  |  Kaufmann, D., et al. 1999. Biol Chem. 380: 1071-8. PMID: 10543444
2. Molecular dissection of isolated disease features in mosaic neurofibromatosis type 1.  |  Maertens, O., et al. 2007. Am J Hum Genet. 81: 243-51. PMID: 17668375
3. Regulation of neuron-specific alternative splicing of neurofibromatosis type 1 pre-mRNA.  |  Zhu, H., et al. 2008. Mol Cell Biol. 28: 1240-51. PMID: 18086893
4. RAS signaling in colorectal carcinomas through alteration of RAS, RAF, NF1, and/or RASSF1A.  |  Ahlquist, T., et al. 2008. Neoplasia. 10: 680-6, 2 p following 686. PMID: 18592002
5. Identification of the neurofibromatosis type 1 gene product.  |  Gutmann, DH., et al. 1991. Proc Natl Acad Sci U S A. 88: 9658-62. PMID: 1946382
6. The neurofibromatosis type 1 gene encodes a protein related to GAP.  |  Xu, GF., et al. 1990. Cell. 62: 599-608. PMID: 2116237
7. The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae.  |  Xu, GF., et al. 1990. Cell. 63: 835-41. PMID: 2121369
8. The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21.  |  Martin, GA., et al. 1990. Cell. 63: 843-9. PMID: 2121370
9. Neurofibromin 1 is a miRNA target in neurons.  |  Paschou, M. and Doxakis, E. 2012. PLoS One. 7: e46773. PMID: 23056445
10. Multipoint linkage analysis in neurofibromatosis type I: an international collaboration.  |  Goldgar, DE., et al. 1989. Am J Hum Genet. 44: 6-12. PMID: 2491784
11. Neurofibromin Regulates Seizure Attacks in the Rat Pilocarpine-Induced Model of Epilepsy.  |  Ren, M., et al. 2016. Mol Neurobiol. 53: 6069-6077. PMID: 26537900
12. The neurofibromin recruitment factor Spred1 binds to the GAP related domain without affecting Ras inactivation.  |  Dunzendorfer-Matt, T., et al. 2016. Proc Natl Acad Sci U S A. 113: 7497-502. PMID: 27313208

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Neurofibromin Antibody (H-12) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Neurofibromin Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Neurofibromin Antibody (H-12) Specifications

Alternate Product Name: Neurofibromin

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-540

Epitope Target: Neurofibromin

Epitope Species: human

Product Reactivity/Detection: Neurofibromin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Neurofibromin Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin