Neuregulin-1/NRG1 Double Nickase Plasmid (h): sc-400352-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neuregulin-1/NRG1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Neuregulin-1/NRG1 Double Nickase Plasmid (h) and Neuregulin-1/NRG1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NRG1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neuregulin-1/NRG1 Antibody (E-12): sc-393006
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neuregulin-1/NRG1 Double Nickase Plasmid (h) | sc-400352-NIC | 20 µg | $410.00 | |||
Neuregulin-1/NRG1 Double Nickase Plasmid (h2) | sc-400352-NIC-2 | 20 µg | $410.00 |
NRG1 encodes neuregulin-1, a membrane-tethered and secreted EGF-like ligand that signals primarily through ERBB3/ERBB4 receptor tyrosine kinases to activate PI3K–AKT and MAPK/ERK pathways. It regulates neuronal development and synaptic plasticity, Schwann cell–axon interactions during peripheral myelination, and cardiomyocyte growth and stress responses. Alternative splicing generates multiple isoforms with distinct extracellular domains and modes of juxtacrine or paracrine signaling, enabling context-specific control of differentiation, migration, and survival programs. Dysregulated NRG1–ERBB signaling has been linked to neuropsychiatric phenotypes, altered myelination, and oncogenic pathway remodeling, making it a valuable target for mechanistic studies of cell communication and receptor signaling.
Neuregulin-1/NRG1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NRG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NRG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NRG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NRG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.