Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Neu2 Double Nickase Plasmid (h): sc-408479-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neu2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Neu2 Double Nickase Plasmid (h) and Neu2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NEU2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neu2 Antibody (37Y): sc-100570
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neu2 Double Nickase Plasmid (h) | sc-408479-NIC | 20 µg | $410.00 | |||
Neu2 Double Nickase Plasmid (h2) | sc-408479-NIC-2 | 20 µg | $410.00 |
Human NEU2 encodes cytosolic neuraminidase 2 (Neu2), a sialidase that removes terminal sialic acids from glycoproteins and glycolipids, thereby modulating the composition and turnover of cellular glycoconjugates. By regulating sialylation status, NEU2 influences glycan-dependent signaling, membrane dynamics, and carbohydrate metabolism processes that intersect with pathways controlling differentiation and stress responses. Altered sialidase activity and aberrant sialylation patterns have been associated with dysregulated cell–cell interactions and oncogenic phenotypes, making NEU2 a useful target for studying how glycosylation remodeling impacts disease-relevant biology. Functional interrogation of NEU2 supports mechanistic research into glycan processing networks and their roles in cellular homeostasis.
Neu2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NEU2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NEU2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NEU2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NEU2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.