Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Neu1 Double Nickase Plasmid (h): sc-401488-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neu1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Neu1 Double Nickase Plasmid (h) and Neu1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NEU1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neu1 Antibody (F-8): sc-166824
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neu1 Double Nickase Plasmid (h) | sc-401488-NIC | 20 µg | $410.00 | |||
Neu1 Double Nickase Plasmid (h2) | sc-401488-NIC-2 | 20 µg | $410.00 |
NEU1 encodes neuraminidase 1 (Neu1), a lysosomal sialidase that removes terminal sialic acids from glycoproteins and glycolipids, contributing to glycoconjugate turnover and lysosome function. Neu1 operates within a multienzyme complex with protective protein/cathepsin A and β-galactosidase, coupling desialylation to broader lysosomal catabolic processes and glycan remodeling. Through regulation of cell-surface sialylation states, NEU1 influences receptor trafficking, endocytosis, and immune-related signaling events that depend on glycoprotein maturation. Perturbation of NEU1 activity is linked to lysosomal storage pathology and altered inflammatory and metabolic phenotypes, making it relevant for mechanistic studies of lysosome-dependent homeostasis.
Neu1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NEU1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NEU1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NEU1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NEU1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.