NELF Double Nickase Plasmid (h): sc-408213-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NELF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NELF Double Nickase Plasmid (h) and NELF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NSMF. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NELF Double Nickase Plasmid (h) | sc-408213-NIC | 20 µg | $410.00 | |||
NELF Double Nickase Plasmid (h2) | sc-408213-NIC-2 | 20 µg | $410.00 |
NSMF encodes NELF, a neuron-expressed, developmentally regulated protein implicated in the guidance and migration of gonadotropin-releasing hormone (GnRH) neurons and the maturation of olfactory and reproductive neuroendocrine circuits. NELF-associated processes intersect with neurodevelopmental signaling programs that coordinate neuronal motility, axonal pathfinding, and tissue-specific transcriptional states during embryogenesis. Genetic variation in NSMF has been linked to disorders of pubertal development and congenital hypogonadotropic hypogonadism, frequently discussed in the context of anosmia-related phenotypes. These associations make NSMF a relevant target for mechanistic studies of neuronal differentiation, migration dynamics, and gene regulatory networks in human cellular models.
NELF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NSMF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NSMF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NSMF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NSMF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.