Nek4 Antibody (2631C1a) recommended for detection of Nek4 of mouse, rat and human origin by WB and IP

Nek4 Antibody (2631C1a): sc-81332

Nek4 Antibody (2631C1a) is rated 3.0 out of 5 by 1.
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Datasheets

    • Anti-Nek4 Antibody (2631C1a) is a mouse monoclonal IgG1 Nek4 antibody provided at 100 µg/ml
    • raised against a recombinant protein corresponding to an internal region of Nek4 of human origin
    • recommended for detection of Nek4 of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nek4 (2631C1a): sc-81332.

I am using Nek4 (2631C1a): sc-81332 antibody for immunoprecipitation, how much antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2014-06-26
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