Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NEDD8 Double Nickase Plasmid (h): sc-417924-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NEDD8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NEDD8 Double Nickase Plasmid (h) and NEDD8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NEDD8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NEDD8 Antibody (H-2): sc-373741
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEDD8 Double Nickase Plasmid (h) | sc-417924-NIC | 20 µg | $410.00 | |||
NEDD8 Double Nickase Plasmid (h2) | sc-417924-NIC-2 | 20 µg | $410.00 |
NEDD8 encodes a ubiquitin-like modifier that is covalently conjugated to substrates in the neddylation pathway, most prominently cullin family proteins that scaffold cullin–RING E3 ubiquitin ligases. NEDD8 conjugation regulates protein turnover, cell-cycle progression, DNA replication and repair, and stress signaling by modulating CRL activity and downstream ubiquitin-dependent proteostasis. Perturbation of neddylation alters checkpoint control, genome stability, and transcriptional programs, linking NEDD8 pathway dysregulation to proliferative phenotypes and signaling rewiring observed in multiple disease contexts. As a central node in ubiquitin-like post-translational modification, NEDD8 is widely studied in proteomics, pathway mapping, and functional genomics screens.
NEDD8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NEDD8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NEDD8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NEDD8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NEDD8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.