Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NDUFB5 Double Nickase Plasmid (m): sc-425783-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDUFB5 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NDUFB5 Double Nickase Plasmid (m) and NDUFB5 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ndufb5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDUFB5 Antibody (F-2): sc-514245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDUFB5 Double Nickase Plasmid (m) | sc-425783-NIC | 20 µg | $410.00 |
Ndufb5 encodes NDUFB5, an accessory subunit of mitochondrial respiratory chain complex I (NADH:ubiquinone oxidoreductase) that supports efficient electron transfer from NADH to ubiquinone and contributes to oxidative phosphorylation. Proper complex I assembly and stability help maintain mitochondrial membrane potential, ATP production, and redox homeostasis, linking NDUFB5 function to cellular energy metabolism and reactive oxygen species control. In mouse models, perturbation of complex I components is commonly used to study mitochondrial dysfunction in bioenergetic stress responses, metabolic adaptation, and cell death pathways. As a nuclear-encoded complex I factor, NDUFB5 is relevant to research on mitochondrial disease mechanisms and broader phenotypes associated with impaired oxidative phosphorylation.
NDUFB5 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ndufb5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ndufb5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ndufb5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ndufb5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.