NDUFB5 Double Nickase Plasmid (h): sc-410902-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDUFB5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NDUFB5 Double Nickase Plasmid (h) and NDUFB5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NDUFB5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDUFB5 Antibody (F-2): sc-514245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDUFB5 Double Nickase Plasmid (h) | sc-410902-NIC | 20 µg | $410.00 | |||
NDUFB5 Double Nickase Plasmid (h2) | sc-410902-NIC-2 | 20 µg | $410.00 |
NDUFB5 encodes an accessory subunit of mitochondrial respiratory chain complex I (NADH:ubiquinone oxidoreductase) that contributes to proper complex assembly and electron transfer from NADH to ubiquinone. Through its role in oxidative phosphorylation, NDUFB5 influences mitochondrial membrane potential maintenance, ATP production, and cellular redox balance, with downstream effects on reactive oxygen species homeostasis. Altered complex I function is linked to mitochondrial bioenergetic stress, metabolic reprogramming, and vulnerabilities in high-energy-demand tissues, making NDUFB5 a relevant node for studying mitochondrial dysfunction. Research on NDUFB5 commonly intersects with pathways controlling mitochondrial proteostasis, mitophagy, and nuclear–mitochondrial signaling responses to impaired respiration.
NDUFB5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NDUFB5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NDUFB5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NDUFB5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NDUFB5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.