NDSB-195 is a member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, to isolate nuclear proteins, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to facilitate the renaturation of chemically and thermally denatured proteins and prevent protein aggregation. NDSBs can be easily removed by dialysis.
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