NCK1 Antibody (20B.1H9): sc-20026

NCK1 Antibody (20B.1H9) is a mouse monoclonal IgG1 kappa light chain antibody that detects NCK1 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). NCK1 (20B.1H9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NCK1, also known as NCK Adaptor Protein 1, plays a crucial role in cellular signaling by acting as an adaptor that links tyrosine kinase receptors, such as the epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) receptors, to downstream signaling pathways. This interaction is vital for regulating processes such as cell proliferation, migration, and cytoskeletal organization. Notably, the SH3 domain of NCK1 binds to proline-rich sequences in proteins like PAK, which is essential for actin cytoskeleton dynamics. Dysregulation of NCK1 can lead to altered cellular responses to growth factors, impacting various physiological and pathological processes, including cancer progression. The human NCK1 gene is located on chromosome 3q21, highlighting its significance in genetic studies related to cell signaling and disease.

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NCK1 Antibody (20B.1H9) References:

1. Nckbeta adapter regulates actin polymerization in NIH 3T3 fibroblasts in response to platelet-derived growth factor bb.  |  Chen, M., et al. 2000. Mol Cell Biol. 20: 7867-80. PMID: 11027258
2. A dominant-negative p65 PAK peptide inhibits angiogenesis.  |  Kiosses, WB., et al. 2002. Circ Res. 90: 697-702. PMID: 11934838
3. The Nck family of adapter proteins: regulators of actin cytoskeleton.  |  Buday, L., et al. 2002. Cell Signal. 14: 723-31. PMID: 12034353
4. Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP.  |  Park, D. and Rhee, SG. 1992. Mol Cell Biol. 12: 5816-23. PMID: 1333046
5. Downregulation of Nck1 After Spinal Cord Injury in Adult Rats.  |  Yang, R., et al. 2022. Balkan Med J. 39: 39-47. PMID: 34928232
6. Complementary Nck1/2 Signaling in Podocytes Controls α Actinin-4-Mediated Actin Organization, Adhesion, and Basement Membrane Composition.  |  Martin, CE., et al. 2022. J Am Soc Nephrol. 33: 1546-1567. PMID: 35906089
7. LncRNA NCK1-AS1-mediated regulatory functions in human diseases.  |  Wang, Y., et al. 2023. Clin Transl Oncol. 25: 323-332. PMID: 36131072
8. Nck1 activity in lateral amygdala regulates long-term fear memory formation.  |  Ilovich, O., et al. 2022. Transl Psychiatry. 12: 475. PMID: 36371406
9. NCK1 Modulates Neuronal Actin Dynamics and Promotes Dendritic Spine, Synapse, and Memory Formation.  |  Diab, AM., et al. 2023. J Neurosci. 43: 885-901. PMID: 36535770
10. A review on the role of NCK1 Antisense RNA 1 (NCK1-AS1) in diverse disorders.  |  Taheri, M., et al. 2023. Pathol Res Pract. 245: 154451. PMID: 37028107
11. Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2).  |  Huebner, K., et al. 1994. Genomics. 22: 281-7. PMID: 7806213
12. Identification of Nck family genes, chromosomal localization, expression, and signaling specificity.  |  Chen, M., et al. 1998. J Biol Chem. 273: 25171-8. PMID: 9737977

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NCK1 Antibody (20B.1H9) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-NCK1 Antibody (20B.1H9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NCK1 Antibody (20B.1H9) Specifications

Alternate Product Name: NCK

Product Clone Name: 20B.1H9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: NCK1

Epitope Species: human

Product Reactivity/Detection: NCK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for NCK1 Antibody (20B.1H9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin