NBR1 Double Nickase Plasmid (h): sc-402592-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NBR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NBR1 Double Nickase Plasmid (h) and NBR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NBR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NBR1 Antibody (4BR): sc-130380
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NBR1 Double Nickase Plasmid (h) | sc-402592-NIC | 20 µg | $410.00 | |||
NBR1 Double Nickase Plasmid (h2) | sc-402592-NIC-2 | 20 µg | $410.00 |
NBR1 (neighbor of BRCA1 gene 1) encodes a selective autophagy receptor that couples ubiquitinated cargo to the autophagosome via its LC3-interacting region and ubiquitin-binding domains. It functions alongside related receptors such as SQSTM1/p62 to support proteostasis by directing protein aggregates, damaged organelles, and invading particles toward lysosomal degradation. Through roles in aggrephagy and pexophagy, NBR1 contributes to cellular stress responses and intersects with pathways that regulate oxidative stress and inflammatory signaling. Dysregulated NBR1-dependent cargo clearance has been implicated in disorders characterized by defective autophagy and protein quality control, including neurodegeneration and cancer-associated stress adaptation.
NBR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NBR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NBR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NBR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NBR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.