NBR1 Double Nickase Plasmid (m): sc-421827-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NBR1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NBR1 Double Nickase Plasmid (m) and NBR1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nbr1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NBR1 Antibody (4BR): sc-130380
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NBR1 Double Nickase Plasmid (m) | sc-421827-NIC | 20 µg | $410.00 | |||
NBR1 Double Nickase Plasmid (m2) | sc-421827-NIC-2 | 20 µg | $410.00 |
Mouse Nbr1 encodes NBR1, a selective autophagy receptor that recognizes ubiquitinated cargo through its UBA domain and engages LC3/GABARAP proteins via an LIR motif to promote autophagosome targeting. NBR1 functions alongside p62/SQSTM1 in aggrephagy and contributes to proteostasis, organelle quality control, and stress responses that shape cell survival and inflammatory signaling. Through interactions with ubiquitin-dependent trafficking and autophagy–lysosome pathways, NBR1 influences turnover of protein aggregates and damaged cellular components. Dysregulation of these processes is relevant to research on neurodegeneration, metabolic stress, and tumor biology where impaired autophagic flux and aberrant ubiquitin signaling are common features.
NBR1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nbr1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nbr1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nbr1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nbr1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.