Date published: 2026-5-22

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Nanog Antibody (J-29): sc-81961

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Datasheets
  • Nanog Antibody (J-29) is a mouse monoclonal IgG2b κ, cited in 7 publications, provided at 100 µg/ml
  • raised against recombinant Nanog of human origin
  • recommended for detection of Nanog of human origin by WB, IP and ELISA
  • See Nanog (1E6C4): sc-293121 for Nanog antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nanog Antibody (J-29). This work is in progress.
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Nanog Antibody (J-29) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize the human Nanog protein, a pivotal transcription factor integral to maintaining the pluripotency and self-renewal of embryonic stem cells. Targeting the recombinant human Nanog epitope, anti-Nanog antibody (J-29) is validated for use in various applications including western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA), ensuring robust and reliable detection of Nanog in human-origin samples. Nanog, also called Nanog homeobox, plays a critical role in regulating gene expression programs that sustain the undifferentiated state of pluripotent stem cells, making it essential for stem cell research and the advancement of regenerative medicine. By maintaining the balance between stem cell renewal and differentiation, Nanog is fundamental to embryonic development and cellular reprogramming processes. Nanog monoclonal antibody (J-29) facilitates detailed studies into these mechanisms, enabling researchers to explore the molecular underpinnings of stem cell biology, developmental biology, and potential therapeutic applications. Anti-Nanog antibody (J-29) is an invaluable tool for scientists investigating the pathways that govern cellular pluripotency and differentiation, ultimately contributing to breakthroughs in understanding and manipulating stem cell fate.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Nanog Antibody (J-29)

sc-81961
100 µg/ml
$339.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with Nanog (J-29): sc-81961 antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
  • y_2026, m_5, d_21, h_10CST
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Rated 5 out of 5 by from WB published data with human tongue cancerWB published data with human tongue cancer cell line SCC25 -SCBT Publication Review
Date published: 2015-06-02
Rated 5 out of 5 by from Antibody was published for use in WB andAntibody was published for use in WB and IF on human OSCC cell lines (SAS and OECM-1) - SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Produced a clear bands with HeLa nuclear extractProduced a clear bands with HeLa nuclear extract. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from This monoclonal worked much better with ourThis monoclonal worked much better with our human samples, and we will buy again.
Date published: 2015-03-25
Rated 5 out of 5 by from Good WB publishable data gathered with HUVECsGood WB publishable data gathered with HUVECs, human pulmonary arterial ECs (HPAECs), and HSaVECs. -SCBT Publication Review
Date published: 2014-05-23
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Nanog Antibody (J-29) is rated 5.0 out of 5 by 5.
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