Nalpha-Acetyl-L-lysine 4-nitroanilide hydrochloride (CAS 50931-35-6 (non-salt))

Nalpha-Acetyl-L-lysine 4-nitroanilide hydrochloride is a chemical compound of significance in various scientific and research applications. It belongs to the class of compounds known as peptide derivatives. This compound has been widely employed in biochemical studies and enzymatic assays. Nalpha-Acetyl-L-lysine 4-nitroanilide hydrochloride serves as a substrate or a chromogenic reagent for specific enzymes, allowing for their detection, quantification, and characterization. It exhibits unique chemical properties that make it suitable for enzymatic analysis and investigation. The compound′s molecular structure combines Nalpha-acetyl-L-lysine and 4-nitroanilide, conferring specific chemical properties and reactivity. These attributes make it valuable in enzymology and proteomics research, as it can be utilized to probe the activity and specificity of enzymes that interact with lysine-containing substrates. Nalpha-Acetyl-L-lysine 4-nitroanilide hydrochloride provides a sensitive and reliable means for monitoring enzyme-catalyzed reactions, as the enzymatic activity leads to the release of a chromogenic product that can be measured spectrophotometrically. This property enables researchers to quantify enzyme activity and evaluate the impact of various factors, such as inhibitors or activators, on enzyme function. Overall, Nalpha-Acetyl-L-lysine 4-nitroanilide hydrochloride serves as a versatile tool in enzymology and biochemical research, enabling the investigation of enzyme kinetics, substrate specificity, and modulation of enzymatic activity. Its use in various experimental setups has contributed to advancing our understanding of enzymatic processes and has potential applications in drug discovery and development.