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Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide (CAS 95713-52-3)

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Alternate Names:
Marfey′s reagent
Application:
Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide is a reagent for the assay of enantiomeric purity of amino-acids with HPLC
CAS Number:
95713-52-3
Purity:
≥98%
Molecular Weight:
272.19
Molecular Formula:
C9H9FN4O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Researchers Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide in post-synthesis modification of peptides, which is critical in the study of protein interactions and dynamics. Its application extends to the field of proteomics, where understanding protein composition, structure, and activity within complex biological systems is the focus. It plays a role in examining metabolic pathways and is used to detect and measure small molecules. This compound plays a role in the study of protein structure and function, as it helps in the mapping of peptide sequences by tagging amino groups in the peptide chain.


Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide (CAS 95713-52-3) References

  1. UV-Guided isolation of verrucines A and B, novel quinazolines from penicillium verrucosum structurally related to anacine from penicilliumaurantiogriseum.  |  Larsen, TO., et al. 1999. J Nat Prod. 62: 1578-80. PMID: 10579880
  2. Analysis of O-phosphoamino acids in biological samples by gas chromatography with flame photometric detection.  |  Kataoka, H., et al. 2000. Methods Mol Biol. 159: 183-206. PMID: 11050725
  3. Enantioselective synthesis and application of the highly fluorescent and environment-sensitive amino acid 6-(2-dimethylaminonaphthoyl) alanine (DANA).  |  Nitz, M., et al. 2002. Chem Commun (Camb). 1912-3. PMID: 12271671
  4. Acid hydrolysis of silk fibroins and determination of the enrichment of isotopically labeled amino acids using precolumn derivatization and high-performance liquid chromatography-electrospray ionization-mass spectrometry.  |  Hess, S., et al. 2002. Anal Biochem. 311: 19-26. PMID: 12441148
  5. Petrosifungins A and B, novel cyclodepsipeptides from a sponge-derived strain of Penicillium brevicompactum.  |  Bringmann, G., et al. 2004. J Nat Prod. 67: 311-5. PMID: 15043401
  6. Chirality determination of unusual amino acids using precolumn derivatization and liquid chromatography-electrospray ionization mass spectrometry.  |  Hess, S., et al. 2004. J Chromatogr A. 1035: 211-9. PMID: 15124814
  7. N-methylphenylalanyl-dehydrobutyrine diketopiperazine, an A-factor mimic that restores antibiotic biosynthesis and morphogenesis in Streptomyces globisporus 1912-B2 and Streptomyces griseus 1439.  |  Matselyukh, B., et al. 2015. J Antibiot (Tokyo). 68: 9-14. PMID: 25005816
  8. Two New Cyclic Depsipeptides from the Endophytic Fungus Fusarium sp.  |  Lv, F., et al. 2015. Nat Prod Commun. 10: 1667-70. PMID: 26669100
  9. Chiral Analysis of Methamphetamine in Oral Fluid Samples: A Method to Distinguish Licit from Illicit Drug Use.  |  Borg, D., et al. 2018. J Anal Toxicol. 42: 25-32. PMID: 29036309
  10. Development of an integrated chromatographic system for ω-transaminase-IMER characterization useful for flow-chemistry applications.  |  Corti, M., et al. 2019. J Pharm Biomed Anal. 169: 260-268. PMID: 30884324
  11. A sensitive HPLC-MS/MS method for the detection, resolution and quantitation of cathinone enantiomers in horse blood plasma and urine.  |  Loganathan, D., et al. 2021. Anal Bioanal Chem. 413: 2147-2161. PMID: 33517480
  12. Chiral derivatization-enabled discrimination and on-tissue detection of proteinogenic amino acids by ion mobility mass spectrometry.  |  Xie, C., et al. 2022. Chem Sci. 13: 14114-14123. PMID: 36540812
  13. Separation and quantification of D- and L-phosphoserine in rat brain using N alpha-(2,4-dinitro-5-fluorophenyl)-L-alaninamide (Marfey's reagent) by high-performance liquid chromatography with ultraviolet detection.  |  Goodnough, DB., et al. 1995. J Chromatogr B Biomed Appl. 672: 290-4. PMID: 8581136

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, 25 mg

sc-253171
25 mg
$30.00

Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, 250 mg

sc-253171A
250 mg
$163.00

Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, 1 g

sc-253171B
1 g
$245.00

Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, 5 g

sc-253171C
5 g
$989.00

Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, 25 g

sc-253171D
25 g
$4600.00