NALP10 Double Nickase Plasmid (m): sc-434050-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NALP10 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NALP10 Double Nickase Plasmid (m) and NALP10 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nlrp10. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NALP10 Double Nickase Plasmid (m) | sc-434050-NIC | 20 µg | $410.00 |
Mouse Nlrp10 encodes NALP10 (NLRP10), a member of the NOD-like receptor family implicated in innate immune regulation and inflammasome-related signaling. Although NLRP10 lacks a canonical C-terminal LRR domain, it has been linked to control of inflammatory responses through modulation of NF-κB-associated pathways, cytokine output, and myeloid and dendritic cell functions that shape adaptive immunity. Nlrp10 activity intersects with pattern-recognition receptor networks that influence leukocyte activation, antigen presentation, and mucosal immune homeostasis. Dysregulation of NLR pathway components is broadly relevant to inflammatory disease mechanisms, making Nlrp10 a useful locus for mechanistic studies of immune signaling and immune cell differentiation in mouse models.
NALP10 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nlrp10 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nlrp10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nlrp10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nlrp10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.