N1-(2,4-Dinitro-phenyl)-ethane-1,2-diamine (CAS 28767-75-1)

N1-(2,4-Dinitro-phenyl)-ethane-1,2-diamine (DNPE) is a widely utilized chemical compound in scientific research due to its remarkable ability to interact with amino acids and peptides. This yellow crystalline powder is synthesized by nitrating 2,4-dinitrophenol and subsequently reacting it with ethylenediamine. In scientific research, N1-(2,4-Dinitro-phenyl)-ethane-1,2-diamine holds significant prominence for its capacity to react with amino acids and peptides. It is employed as a valuable reagent in the determination of amino acids and peptides within biological samples. Moreover, this compound plays a pivotal role in the synthesis of peptides and derivatives of amino acids. The reaction of N1-(2,4-Dinitro-phenyl)-ethane-1,2-diamine with amino acids and peptides follows a nucleophilic substitution process. In this process, the amino group of the amino acid or peptide replaces the nitro group of N1-(2,4-Dinitro-phenyl)-ethane-1,2-diamine, resulting in the formation of a distinct yellow-colored product. This reaction exhibits high specificity for primary amines, and the resulting product can be easily quantified using spectrophotometric techniques.