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N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is an oxidizable compound that serves as a reducing co-substrate for heme peroxidases. N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm. Thus, the stoichiometry of oxidation is 2 moles of N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride at 611 nm is 12,200. N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride is also used for the detection of peroxidases on polyacrylamide gels.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride, 5 g | sc-205528 | 5 g | $68.00 | |||
N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride, 10 g | sc-205528A | 10 g | $111.00 |