Date published: 2026-4-4

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N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (CAS 637-01-4)

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Alternate Names:
TMPD dihydrochloride
CAS Number:
637-01-4
Purity:
98%
Molecular Weight:
237.17
Molecular Formula:
C10H16N22HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is an oxidizable compound that serves as a reducing co-substrate for heme peroxidases. N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm. Thus, the stoichiometry of oxidation is 2 moles of N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride at 611 nm is 12,200. N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride is also used for the detection of peroxidases on polyacrylamide gels.


N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (CAS 637-01-4) References

  1. The respiratory system and diazotrophic activity of Acetobacter diazotrophicus PAL5.  |  Flores-Encarnación, M., et al. 1999. J Bacteriol. 181: 6987-95. PMID: 10559164
  2. Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion.  |  Kuznetsov, AV., et al. 2004. Am J Physiol Heart Circ Physiol. 286: H1633-41. PMID: 14693685
  3. Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans.  |  Cavalheiro, RA., et al. 2004. Braz J Med Biol Res. 37: 1455-61. PMID: 15448865
  4. The presence of oxidized phosphatidylserine on Fas-mediated apoptotic cell surface.  |  Matsura, T., et al. 2005. Biochim Biophys Acta. 1736: 181-8. PMID: 16168707
  5. Effects of thyroid hormones on the bypasses of the antimycin A block in the bc1 complex of rat liver mitochondria.  |  Horrum, MA., et al. 1991. Biochem Biophys Res Commun. 178: 73-8. PMID: 1648915
  6. Inhibition of cytochrome oxidase by dibucaine.  |  Stringer, BK. and Harmon, HJ. 1990. Biochem Pharmacol. 40: 1077-81. PMID: 2167679
  7. Remote and local ischemic preconditioning equivalently protects rat skeletal muscle mitochondrial function during experimental aortic cross-clamping.  |  Mansour, Z., et al. 2012. J Vasc Surg. 55: 497-505.e1. PMID: 22056287
  8. Polyphenols prevent ageing-related impairment in skeletal muscle mitochondrial function through decreased reactive oxygen species production.  |  Charles, AL., et al. 2013. Exp Physiol. 98: 536-45. PMID: 22903980
  9. Pretreatment with brain natriuretic peptide reduces skeletal muscle mitochondrial dysfunction and oxidative stress after ischemia-reperfusion.  |  Talha, S., et al. 2013. J Appl Physiol (1985). 114: 172-9. PMID: 23104692
  10. Analysis of sulfonamides, trimethoprim, fluoroquinolones, quinolones, triphenylmethane dyes and methyltestosterone in fish and shrimp using liquid chromatography-mass spectrometry.  |  Storey, JM., et al. 2014. J Chromatogr B Analyt Technol Biomed Life Sci. 972: 38-47. PMID: 25310706
  11. Propofol-induced mitochondrial and contractile dysfunction of the rat ventricular myocardium.  |  Grundmanová, M., et al. 2016. Physiol Res. 65: S601-S609. PMID: 28006942
  12. Repair of tryptophan radicals by antioxidants.  |  Jovanovic, SV. and Simic, MG. 1985. J Free Radic Biol Med. 1: 125-9. PMID: 3836239
  13. Glucose transport in isolated prosthecae of Asticcacaulis biprosthecum.  |  Larson, RJ. and Pate, JL. 1976. J Bacteriol. 126: 282-93. PMID: 4425

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride, 5 g

sc-205528
5 g
$68.00

N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride, 10 g

sc-205528A
10 g
$111.00