N-Butylfluorescein (CAS 335193-91-4)

N-Butylfluorescein (NBF) stands as a fluorescent dye extensively employed in numerous scientific applications, encompassing cell imaging, protein labeling, and DNA sequencing. This small, water-soluble molecule exhibits a high fluorescence quantum yield. Additionally, N-Butylfluorescein finds utility in protein and nucleic acid detection, as well as enzyme activity measurement. It serves as a valuable fluorescent probe for detecting proteins and nucleic acids, enabling their visualization. Furthermore, N-Butylfluorescein aids in measuring enzyme activity and contributes to the study of cell membrane dynamics, gene expression, and protein-protein interactions. The functioning of N-Butylfluorescein as a fluorescent dye relies on its excitation by light of a specific wavelength. Upon excitation, the dye emits light of a longer wavelength, which can be detected by appropriate instruments like fluorescence microscopes or spectrophotometers. Moreover, N-Butylfluorescein can be utilized to label proteins or nucleic acids, enabling their visualization under a microscope.