N-4-Tosyl-glycyl-L-prolyl-L-arginine 7-amido-4-methylcoumarin hydrochloride (CAS 117961-27-0)

N-4-Tosyl-glycyl-L-prolyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is employed in biochemical research, particularly in the study of protease activity. This compound is a fluorogenic peptide substrate, with its structure designed to be specifically cleaved by certain proteases, such as thrombin. Upon enzymatic cleavage, it releases a fluorescent 7-amido-4-methylcoumarin moiety, which can be detected and quantified using fluorescence spectroscopy. This feature makes it a useful tool for monitoring enzyme kinetics and for identifying protease inhibitors in a high-throughput screening format. The specificity of N-4-Tosyl-glycyl-L-prolyl-L-arginine 7-amido-4-methylcoumarin hydrochloride for particular proteases provides valuable information about the substrate preferences and catalytic mechanisms of these enzymes, contributing to the broader understanding of their roles in biological processes. Additionally, the hydrochloride salt form enhances the solubility of the compound, facilitating its use in various experimental conditions.