N-(4-Carboxy-3-hydroxyphenyl)maleimide (CAS 19232-43-0)

N-(4-Carboxy-3-hydroxyphenyl)maleimide (NCHPMA) is an hydrophilic, water-soluble, and biocompatible compound that serves as an irreversible inhibitor of LDH isoenzyme. Widely used in scientific research and laboratory experiments, N-(4-Carboxy-3-hydroxyphenyl)maleimide exhibits a broad range of applications. This versatile compound acts as a valuable reagent for various biochemical and physiological studies. The applications of N-(4-Carboxy-3-hydroxyphenyl)maleimide extend to the field of scientific research and laboratory experiments, making it an adaptable compound for diverse studies. It serves as a fluorescent probe, facilitating the detection of proteins, enzymes, and nucleic acids. Furthermore, it proves instrumental in the examination of protein-protein interactions, DNA-protein interactions, as well as the exploration of protein structure and function. By binding to proteins, enzymes, and nucleic acids, N-(4-Carboxy-3-hydroxyphenyl)maleimide actively engages with the target molecules. This interaction occurs through the formation of covalent bonds with the amino acid residues of the target molecule. Stabilized by hydrogen bonds and van der Waals forces, the resulting covalent bond enables N-(4-Carboxy-3-hydroxyphenyl)maleimide to function as a fluorescent probe, facilitating the detection of proteins, enzymes, and nucleic acids.