MYH11 Antibody (G-4): sc-6956
- MYH11 Antibody (G-4) is a mouse monoclonal IgG1 λ MYH11 antibody, cited in 72 publications, provided at 200 µg/ml
- raised against full length smooth muscle myosin heavy chain of rat origin
- MYH11 Antibody (G-4) is recommended for detection of myosin heavy chain 11, isoforms SM1 and SM2 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including and bovine and porcine
- Anti-MYH11 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MYH11 Antibody (G-4) for WB applications. These reagents are now offered in bundles with MYH11 Antibody (G-4) (see ordering information below).
MYH11 Antibody (G-4) is a mouse monoclonal IgG1 antibody that detects MYH11 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-MYH11 antibody (G-4) is raised against full length smooth muscle myosin heavy chain of rat origin. MYH11 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MYH11 protein, also known as smooth muscle myosin heavy chain, plays a crucial role in muscle contraction and cellular movement by interacting with actin filaments to generate force. This interaction is vital for various physiological processes, including vasoconstriction and gastrointestinal motility, making MYH11 an important target for studying smooth muscle function and related disorders. The structure of MYH11 consists of a hexameric assembly, which includes two heavy chain subunits and light chain subunits that can be either phosphorylatable or non-phosphorylatable. The phosphorylation state of the myosin light chain is a key regulatory mechanism for smooth muscle contraction, as modulated by calcium/calmodulin-dependent myosin light chain kinase. Understanding the structure and function of MYH11 is essential for elucidating the mechanisms underlying smooth muscle physiology and pathophysiology, as well as for developing therapeutic strategies for conditions involving smooth muscle dysfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MYH11 Antibody (G-4) References:
- Spatial and temporal changes in myosin heavy chain gene expression in skeletal muscle development. | Lu, BD., et al. 1999. Dev Biol. 216: 312-26. PMID: 10588881
- Identification of three developmentally controlled isoforms of human myosin heavy chains. | Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
- Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA. | Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
- Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis. | Nagai, R., et al. 1989. J Biol Chem. 264: 9734-7. PMID: 2722872
- Myh11 Lineage Corneal Endothelial Cells and ASCs Populate Corneal Endothelium. | Corliss, BA., et al. 2019. Invest Ophthalmol Vis Sci. 60: 5095-5103. PMID: 31826231
- Prospective evaluation of prognostic impact of KIT mutations on acute myeloid leukemia with RUNX1-RUNX1T1 and CBFB-MYH11. | Ishikawa, Y., et al. 2020. Blood Adv. 4: 66-75. PMID: 31899799
- Generating endogenous Myh11-driven Cre mice for sex-independent gene deletion in smooth muscle cells. | Zhao, Y., et al. 2023. JCI Insight. 8: PMID: 37289544
- Characterization of a human perinatal myosin heavy-chain transcript. | Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
- Regulation of differentiation of vascular smooth muscle cells. | Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
- Phylogenetic analysis of the myosin superfamily. | Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
- Mechanisms of smooth muscle contraction. | Horowitz, A., et al. 1996. Physiol Rev. 76: 967-1003. PMID: 8874491
- The mammalian myosin heavy chain gene family. | Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYH11 Antibody (G-4) | sc-6956 | 200 µg/ml | $322.00 | |||
MYH11 Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-526487 | 200 µg Ab; 10 µg BP | $361.00 | |||
MYH11 Antibody (G-4): m-IgG1 BP-HRP Bundle | sc-531860 | 200 µg Ab; 20 µg BP | $361.00 | |||
MYH11 Antibody (G-4) AC | sc-6956 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MYH11 Antibody (G-4) HRP | sc-6956 HRP | 200 µg/ml | $322.00 | |||
MYH11 Antibody (G-4) FITC | sc-6956 FITC | 200 µg/ml | $336.00 | |||
MYH11 Antibody (G-4) PE | sc-6956 PE | 200 µg/ml | $349.00 | |||
MYH11 Antibody (G-4) Alexa Fluor® 488 | sc-6956 AF488 | 200 µg/ml | $364.00 | |||
MYH11 Antibody (G-4) Alexa Fluor® 546 | sc-6956 AF546 | 200 µg/ml | $364.00 | |||
MYH11 Antibody (G-4) Alexa Fluor® 594 | sc-6956 AF594 | 200 µg/ml | $364.00 | |||
MYH11 Antibody (G-4) Alexa Fluor® 647 | sc-6956 AF647 | 200 µg/ml | $364.00 | |||
MYH11 Antibody (G-4) Alexa Fluor® 680 | sc-6956 AF680 | 200 µg/ml | $364.00 | |||
MYH11 Antibody (G-4) Alexa Fluor® 790 | sc-6956 AF790 | 200 µg/ml | $364.00 |