MYH11 Antibody (G-4): sc-6956

MYH11 Antibody (G-4) is a mouse monoclonal IgG1 antibody that detects MYH11 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-MYH11 antibody (G-4) is raised against full length smooth muscle myosin heavy chain of rat origin. MYH11 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MYH11 protein, also known as smooth muscle myosin heavy chain, plays a crucial role in muscle contraction and cellular movement by interacting with actin filaments to generate force. This interaction is vital for various physiological processes, including vasoconstriction and gastrointestinal motility, making MYH11 an important target for studying smooth muscle function and related disorders. The structure of MYH11 consists of a hexameric assembly, which includes two heavy chain subunits and light chain subunits that can be either phosphorylatable or non-phosphorylatable. The phosphorylation state of the myosin light chain is a key regulatory mechanism for smooth muscle contraction, as modulated by calcium/calmodulin-dependent myosin light chain kinase. Understanding the structure and function of MYH11 is essential for elucidating the mechanisms underlying smooth muscle physiology and pathophysiology, as well as for developing therapeutic strategies for conditions involving smooth muscle dysfunction.

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MYH11 Antibody (G-4) References:

1. Spatial and temporal changes in myosin heavy chain gene expression in skeletal muscle development.  |  Lu, BD., et al. 1999. Dev Biol. 216: 312-26. PMID: 10588881
2. Identification of three developmentally controlled isoforms of human myosin heavy chains.  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
3. Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA.  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
4. Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis.  |  Nagai, R., et al. 1989. J Biol Chem. 264: 9734-7. PMID: 2722872
5. Myh11 Lineage Corneal Endothelial Cells and ASCs Populate Corneal Endothelium.  |  Corliss, BA., et al. 2019. Invest Ophthalmol Vis Sci. 60: 5095-5103. PMID: 31826231
6. Prospective evaluation of prognostic impact of KIT mutations on acute myeloid leukemia with RUNX1-RUNX1T1 and CBFB-MYH11.  |  Ishikawa, Y., et al. 2020. Blood Adv. 4: 66-75. PMID: 31899799
7. Generating endogenous Myh11-driven Cre mice for sex-independent gene deletion in smooth muscle cells.  |  Zhao, Y., et al. 2023. JCI Insight. 8: PMID: 37289544
8. Characterization of a human perinatal myosin heavy-chain transcript.  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
9. Regulation of differentiation of vascular smooth muscle cells.  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
10. Phylogenetic analysis of the myosin superfamily.  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
11. Mechanisms of smooth muscle contraction.  |  Horowitz, A., et al. 1996. Physiol Rev. 76: 967-1003. PMID: 8874491
12. The mammalian myosin heavy chain gene family.  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MYH11 Antibody (G-4) has 72 citations in a variety of scientific publications.

Primary antibodies like Anti-MYH11 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MYH11 Antibody (G-4) Specifications

Alternate Product Name: MYH11

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids:

Epitope Target: smooth muscle myosin heavy chain

Epitope Species: rat

Product Reactivity/Detection: myosin heavy chain 11, isoforms SM1 and SM2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 72 citations in various scientific publications

Available Conjugates/Forms for MYH11 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin