Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Western blot analysis of c-Myc expression in HeLa (A,D,F), Jurkat (B), K-562 (C) and NIH/3T3 (G) whole cell lysates and Jurkat (E,H) nuclear extracts. Antibodies tested include c-Myc Antibody (9E10): sc-40 (A-C), c-Myc (C-33): sc-42 (D,E) and c-Myc (N-262): sc-764 (F-H).
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Myc Double Nickase Plasmid (m): sc-421770-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myc Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Myc Double Nickase Plasmid (m) and Myc Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Myc. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myc Antibody (9E10): sc-40
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myc Double Nickase Plasmid (m) | sc-421770-NIC | 20 µg | $410.00 | |||
Myc Double Nickase Plasmid (m2) | sc-421770-NIC-2 | 20 µg | $410.00 |