Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Myc Double Nickase Plasmid (m): sc-421770-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myc Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Myc Double Nickase Plasmid (m) and Myc Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Myc. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myc Antibody (9E10): sc-40
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myc Double Nickase Plasmid (m) | sc-421770-NIC | 20 µg | $410.00 | |||
Myc Double Nickase Plasmid (m2) | sc-421770-NIC-2 | 20 µg | $410.00 |
Myc (c-Myc) is a basic helix–loop–helix leucine zipper transcription factor that heterodimerizes with MAX to regulate broad gene expression programs controlling cell growth, ribosome biogenesis, metabolism, and cell cycle progression. It integrates signals from mitogenic pathways including Wnt/β-catenin, MAPK/ERK, PI3K–AKT–mTOR, and TGF-β, coordinating transcriptional outputs that influence proliferation and differentiation. In mouse models, dysregulated Myc activity perturbs genomic stability, apoptosis, and cellular reprogramming states, making it a central node for studying oncogenic signaling and transcriptional control. Myc-dependent networks are widely used to interrogate context-specific vulnerability in transformed versus normal cells and to map gene regulatory circuits in development and disease biology.
Myc Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Myc locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Myc. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Myc function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Myc-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.