Mx1 (h): 293T Lysate: sc-115203
- human Mx1 transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for Mx1 antibodies
- Suitable control antibody: Mx1/2/3 Antibody (C-1): sc-166412 is recommended as control antibody for monitoring of Mx1 expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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Mx1 (h): 293T Lysate, derived from the HEK 293T cell line, is a crucial reagent for studying the Myxovirus resistance protein 1 (Mx1), an interferon-induced dynamin-like GTPase involved in the cellular antiviral response. The HEK 293T cells, a derivative of the HEK 293 line modified to express the SV40 large T antigen, are optimized for high-efficiency protein expression, making them ideal for producing lysates that contain significant levels of Mx1. Researchers utilize Mx1 (h): 293T Lysate to investigate the mechanisms by which Mx1 mediates its antiviral activities. Mx1 is known to interfere with the replication of various RNA viruses by binding to viral nucleocapsids and disrupting their assembly. Techniques such as immunoprecipitation, Western blotting, and GTPase activity assays are employed to study the interaction of Mx1 with viral components and its subsequent impact on viral replication. This lysate is instrumental in elucidating the signal transduction pathways activated by interferons that lead to the induction of Mx1. By exploring these pathways, scientists can gain a deeper understanding of the cellular defense mechanisms against viral infections.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mx1 (h): 293T Lysate | sc-115203 | 100 µg/200 µl | $232.00 |