Muscle Actin Antibody (HHF35): sc-53014
- Muscle Actin Antibody (HHF35) is a mouse monoclonal IgG1, cited in 3 publications, provided at 200 µg/ml
- raised against myocardium of human origin
- recommended for detection of muscle specific α and γ Actin isomers of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with β Actin isomers
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Muscle Actin Antibody (HHF35). This work is in progress.
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Muscle Actin Antibody (HHF35) is a mouse monoclonal IgG1 antibody that detects Muscle Actin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Muscle Actin antibody (HHF35) plays a crucial role in studying the actin cytoskeleton, which is essential for various cellular functions, including maintaining cell shape, enabling motility, and facilitating intracellular transport. Muscle Actin (HHF35) monoclonal antibody helps examine multiple actin isoforms, with α-Actin being specifically expressed in muscle tissues, while β-Actin and γ-Actin are predominant in non-muscle cells. Dynamic actin filaments rapidly assemble and disassemble, which is vital for processes such as cell division and muscle contraction. Muscle Actin (HHF35) antibody enables research into actin′s interactions with various proteins, including members of the small GTPase family, which are critical for regulating organization and function within cells. Rho, Rac, and Cdc42 are key regulators that influence actin filament dynamics, stress fiber formation, and development of cellular protrusions, respectively. Understanding these interactions and functional implications of actin in cellular processes advances research in muscle biology and related fields.
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Muscle Actin Antibody (HHF35) References:
- Phenotype-dependent expression of alpha-smooth muscle actin in visceral smooth muscle cells. | Saga, H., et al. 1999. Exp Cell Res. 247: 279-92. PMID: 10047470
- Mice lacking skeletal muscle actin show reduced muscle strength and growth deficits and die during the neonatal period. | Crawford, K., et al. 2002. Mol Cell Biol. 22: 5887-96. PMID: 12138199
- Muscle lim protein isoform negatively regulates striated muscle actin dynamics and differentiation. | Vafiadaki, E., et al. 2014. FEBS J. 281: 3261-79. PMID: 24860983
- Osteogenic potential of alpha smooth muscle actin expressing muscle resident progenitor cells. | Matthews, BG., et al. 2016. Bone. 84: 69-77. PMID: 26721734
- Alpha-Smooth Muscle Actin-Positive Perivascular Cells in Diabetic Retina and Choroid. | Kim, SJ., et al. 2020. Int J Mol Sci. 21: PMID: 32245120
- Protocol to detect smooth muscle actin-alpha and measure oxidative damage in neonatal mouse intestine. | Hu, S., et al. 2022. STAR Protoc. 3: 101524. PMID: 35810413
- Role of alpha smooth muscle actin in odontogenic differentiation of dental pulp stem cells. | Ma, Z., et al. 2023. Eur J Oral Sci. 131: e12956. PMID: 37849216
- Dilated cardiomyopathy-associated skeletal muscle actin (ACTA1) mutation R256H disrupts actin structure and function and causes cardiomyocyte hypocontractility. | Garg, A., et al. 2024. Proc Natl Acad Sci U S A. 121: e2405020121. PMID: 39503885
- Muscle coding sequences and their regulation during myogenesis: cloning of muscle actin cDNA probes. | Minty, A., et al. 1981. Reprod Nutr Dev (1980). 21: 247-55. PMID: 6760289
- Smooth muscle actin and myosin expression in cultured airway smooth muscle cells. | Wong, JZ., et al. 1998. Am J Physiol. 274: L786-92. PMID: 9612294
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Muscle Actin Antibody (HHF35) | sc-53014 | 200 µg/ml | $322.00 |