MUM1 Antibody (D-11) recommended for detection of MUM1 of mouse, rat and human origin by WB, IP, IF and ELISA

MUM1 Antibody (D-11): sc-514332

MUM1 Antibody (D-11) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-MUM1 Antibody (D-11) is a mouse monoclonal IgG1 (kappa light chain) MUM1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 72-96 near the N-terminus of MUM1 of human origin
    • Anti-MUM1 Antibody (D-11) is recommended for detection of MUM1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-MUM1 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-514332 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MUM1 (D-11): sc-514332.

IHC(P) isn't listed under recommended detection methods for sc514332. However, it's available conjugated to HRP for IHC(P). Will it or will it not work for IHC(P)? If IHC(P) is an option with this antibody, do you have a recommended dilution range?

Asked by: rescalona
Thank you for your question. We currently do not have data or product citations using this antibody for IHC on formalin-fixed paraffin-embedded tissue sections, and so IHC(P) is not a recommended application at this time. The conjugated primary antibody is recommended and warrantied for IHC(P), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-12-20

What application is the blocking peptide sc-514332 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Works very well. The antibody works fine in U2OS cells in 1:500 dilution.
Date published: 2019-09-27
Rated 5 out of 5 by from Good Western blot data of MUM1 expression in non Good Western blot data of MUM1 expression in non-transfected 293T, mouse MUM1 transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-06-26
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