Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mucin 5AC/MUC5AC Double Nickase Plasmid (m): sc-421752-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mucin 5AC/MUC5AC Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mucin 5AC/MUC5AC Double Nickase Plasmid (m) and Mucin 5AC/MUC5AC Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Muc5ac. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mucin 5AC/MUC5AC Antibody (45M1): sc-21701
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mucin 5AC/MUC5AC Double Nickase Plasmid (m) | sc-421752-NIC | 20 µg | $410.00 |
Muc5ac encodes the gel-forming mucin Mucin 5AC/MUC5AC, a heavily O-glycosylated secreted glycoprotein that is a major structural component of the airway and gastrointestinal mucus barrier in mouse epithelia. Its regulated expression supports mucociliary clearance, epithelial hydration, and innate defense by shaping mucus viscoelastic properties and limiting pathogen contact with the cell surface. Muc5ac is induced during epithelial differentiation and inflammatory remodeling through cytokine- and stress-responsive programs, including EGFR/MAPK signaling and IL-13–STAT6–driven goblet cell metaplasia. Dysregulated Muc5ac production and altered mucin glycosylation are linked to mucus plugging and chronic inflammation in respiratory disease models and to epithelial barrier changes in gastrointestinal pathology, making it a common readout in studies of airway hypersecretion and mucosal immunity.
Mucin 5AC/MUC5AC Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Muc5ac locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Muc5ac. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Muc5ac function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Muc5ac-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.