Mucin 15 Antibody (H-5) recommended for detection of Mucin 15 of mouse, rat and human origin by WB, IP, IF and ELISA

Mucin 15 Antibody (H-5): sc-365746

Mucin 15 Antibody (H-5) is rated 3.5 out of 5 by 2.
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Datasheets

    • Anti-Mucin 15 Antibody (H-5) is a mouse monoclonal IgG1 (kappa light chain) Mucin 15 antibody provided at 200 µg/ml
    • raised against amino acids 23-232 mapping within an N-terminal extracellular domain of Mucin 15 of mouse origin
    • Anti-Mucin 15 Antibody (H-5) is recommended for detection of Mucin 15 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Mucin 15 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mucin 15 (H-5): sc-365746.
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Are there any issues with using sc-365746: Mucin 15 (H-5) mouse monoclonal primary antibody on mouse samples?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-365746 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 2 out of 5 by from Not cross-reactivity with bovine We have tried the antibody on cells isolated from bovine milk and we were unable to detect any binding; thus, we concluded that the antibody does not work on the extracellular region of bovine MUC15.
Date published: 2017-05-22
Rated 5 out of 5 by from Signal via western blot analysis of Mucin Signal via western blot analysis of Mucin 15 expression in non-transfected: sc-117752 (A) and mouse Mucin 15 transfected: sc-127174 (B) 293T whole cell lysates. -SCBT QC
Date published: 2013-09-10
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