MTSEA Biotin (CAS 162758-04-5)

MTSEA Biotin, is a highly versatile biotinylated thiol compound widely employed in the fields of biochemistry and molecular biology. Its exceptional characteristic is its ability to covalently bind to diverse substrates, making it useful for numerous applications. NBME finds extensive use in protein modification, peptide synthesis, DNA/RNA labeling, and enzyme assays. Within scientific research, MTSEA Biotin has played a vital role in proteomics and molecular biology investigations. Its applications span a wide range, from modifying proteins, peptides, and nucleic acids to labeling enzymes and other molecules for diverse assays. Additionally, MTSEA Biotin has enabled the study of protein-protein interactions, the exploration of protein structure and function, as well as investigations into DNA and RNA structure and function. The action of MTSEA Biotin is achieved through its specific covalent binding to various substrates. The biotin moiety of NBME selectively binds to the biotin-binding domains of proteins and other molecules, while the thiol moiety binds to the sulfhydryl groups present in proteins and other molecules. Importantly, this binding process is reversible, allowing the removal of MTSEA Biotin from the substrate using chemical or enzymatic methods.