MtRPOL Antibody (B-1): sc-365082
- MtRPOL Antibody (B-1) is a mouse monoclonal IgG1 κ MtRPOL antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 841-1140 mapping near the C-terminus of MtRPOL of human origin
- MtRPOL Antibody (B-1) is recommended for detection of DNA-directed RNA polymerase, mitochondrial precursor (MtRPOL) of human origin by WB, IP, IF and ELISA
- Anti-MtRPOL Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-365082 X, 200 µg/0.1 ml)
- m-IgG1 BP-HRP is the preferred secondary detection reagent for MtRPOL Antibody (B-1) for WB applications. This reagent is now offered in a bundle with MtRPOL Antibody (B-1) (see ordering information below).
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SEE ALSO...
MtRPOL Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects MtRPOL protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MtRPOL (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The mitochondrial RNA polymerase (MtRPOL) plays a crucial role in the transcription of mitochondrial DNA, which is essential for the proper functioning of mitochondria, the powerhouse of the cell. Mitochondria are responsible for producing adenosine triphosphate (ATP), the energy currency of the cell, and any dysfunction in MtRPOL can lead to impaired energy production, contributing to a range of metabolic disorders and diseases. MtRPOL consists of 1,230 amino acids and exhibits significant homology with mitochondrial RNA polymerases from lower eukaryotes and RNA polymerases from various bacteriophages, highlighting its evolutionary conservation and importance in cellular energy metabolism. Understanding the function and regulation of MtRPOL is vital for elucidating the mechanisms underlying mitochondrial diseases and developing potential therapeutic strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MtRPOL Antibody (B-1) References:
- Discovery of a major D-loop replication origin reveals two modes of human mtDNA synthesis. | Fish, J., et al. 2004. Science. 306: 2098-101. PMID: 15604407
- Transcription of mammalian messenger RNAs by a nuclear RNA polymerase of mitochondrial origin. | Kravchenko, JE., et al. 2005. Nature. 436: 735-9. PMID: 16079853
- Sequence and organization of the human mitochondrial genome. | Anderson, S., et al. 1981. Nature. 290: 457-65. PMID: 7219534
- Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium. | Wasinger, VC., et al. 1995. Electrophoresis. 16: 1090-4. PMID: 7498152
- Polypeptide cartography of Spiroplasma taiwanense. | Humphery-Smith, I., et al. 1994. Electrophoresis. 15: 1212-7. PMID: 7859730
- Identification of the gene encoding the human mitochondrial RNA polymerase (h-mtRPOL) by cyberscreening of the Expressed Sequence Tags database. | Tiranti, V., et al. 1997. Hum Mol Genet. 6: 615-25. PMID: 9097968
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MtRPOL Antibody (B-1) | sc-365082 | 200 µg/ml | $316.00 | |||
MtRPOL Antibody (B-1): m-IgG1 BP-HRP Bundle | sc-531700 | 200 µg Ab; 20 µg BP | $354.00 | |||
MtRPOL Antibody (B-1) X | sc-365082 X | 200 µg/0.1 ml | $316.00 | |||
MtRPOL Antibody (B-1) AC | sc-365082 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MtRPOL Antibody (B-1) HRP | sc-365082 HRP | 200 µg/ml | $316.00 | |||
MtRPOL Antibody (B-1) FITC | sc-365082 FITC | 200 µg/ml | $330.00 | |||
MtRPOL Antibody (B-1) PE | sc-365082 PE | 200 µg/ml | $343.00 | |||
MtRPOL Antibody (B-1) Alexa Fluor® 488 | sc-365082 AF488 | 200 µg/ml | $357.00 | |||
MtRPOL Antibody (B-1) Alexa Fluor® 546 | sc-365082 AF546 | 200 µg/ml | $357.00 | |||
MtRPOL Antibody (B-1) Alexa Fluor® 594 | sc-365082 AF594 | 200 µg/ml | $357.00 | |||
MtRPOL Antibody (B-1) Alexa Fluor® 647 | sc-365082 AF647 | 200 µg/ml | $357.00 | |||
MtRPOL Antibody (B-1) Alexa Fluor® 680 | sc-365082 AF680 | 200 µg/ml | $357.00 | |||
MtRPOL Antibody (B-1) Alexa Fluor® 790 | sc-365082 AF790 | 200 µg/ml | $357.00 |