MTF-1 Antibody (H-6): sc-365090

MTF-1 Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MTF-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MTF-1 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The metal-responsive element (MRE)-binding transcription factor, MTF-1, plays a crucial role in cellular response to heavy metal exposure by stimulating metallothionein expression, which is important for metal detoxification and homeostasis. MTF-1 is primarily located in the nucleus, where MTF-1 binds to specific DNA sequences to regulate gene expression. Nuclear localization allows MTF-1 to effectively respond to changes in metal ion concentrations, thereby influencing cellular stress responses and maintaining metal ion balance. MTF-1′s structure includes six zinc fingers in its DNA-binding domain, which are essential for interaction with target genes. Phosphorylation of MTF-1 upon metal exposure enhances transcriptional activity, further underscoring significance in cellular adaptation to environmental stressors. Beyond metallothionein activation, MTF-1 participates in a post-transcriptional regulatory complex that modulates ribosomal protein S25 mRNA export, highlighting MTF-1′s multifaceted role in cellular regulation.

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MTF-1 Antibody (H-6) References:

1. Ribosomal protein S25 mRNA partners with MTF-1 and La to provide a p53-mediated mechanism for survival or death.  |  Adilakshmi, T. and Laine, RO. 2002. J Biol Chem. 277: 4147-51. PMID: 11741912
2. Regulation of metallothionein transcription by the metal-responsive transcription factor MTF-1: identification of signal transduction cascades that control metal-inducible transcription.  |  Saydam, N., et al. 2002. J Biol Chem. 277: 20438-45. PMID: 11923282
3. Chromatin insulation by a transcriptional activator.  |  Sutter, NB., et al. 2003. Proc Natl Acad Sci U S A. 100: 1105-10. PMID: 12547916
4. Understanding the mechanisms of zinc-sensing by metal-response element binding transcription factor-1 (MTF-1).  |  Laity, JH. and Andrews, GK. 2007. Arch Biochem Biophys. 463: 201-10. PMID: 17462582
5. The metal-responsive transcription factor-1 protein is elevated in human tumors.  |  Shi, Y., et al. 2010. Cancer Biol Ther. 9: 469-76. PMID: 20087061
6. PTEN interacts with metal-responsive transcription factor 1 and stimulates its transcriptional activity.  |  Lin, MC., et al. 2012. Biochem J. 441: 367-77. PMID: 21883094
7. Metal responsive transcription factor 1 (MTF-1) regulates zinc dependent cellular processes at the molecular level.  |  Grzywacz, A., et al. 2015. Acta Biochim Pol. 62: 491-8. PMID: 26336656
8. A zinc-responsive factor interacts with a metal-regulated enhancer element (MRE) of the mouse metallothionein-I gene.  |  Westin, G. and Schaffner, W. 1988. EMBO J. 7: 3763-70. PMID: 3208749
9. The hypoxia sensitive metal transcription factor MTF-1 activates NCX1 brain promoter and participates in remote postconditioning neuroprotection in stroke.  |  Valsecchi, V., et al. 2021. Cell Death Dis. 12: 423. PMID: 33931586
10. Genomic Redistribution of Metal-Response Transcription Factor-1 (MTF-1) in Cadmium Resistant Cells.  |  Wright, GM. and Black, JC. 2023. Cells. 12: PMID: 36980293
11. Cloning, chromosomal mapping and characterization of the human metal-regulatory transcription factor MTF-1.  |  Brugnera, E., et al. 1994. Nucleic Acids Res. 22: 3167-73. PMID: 8065932
12. Cloned transcription factor MTF-1 activates the mouse metallothionein I promoter.  |  Radtke, F., et al. 1993. EMBO J. 12: 1355-62. PMID: 8467794

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MTF-1 Antibody (H-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-MTF-1 Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MTF-1 Antibody (H-6) Specifications

Alternate Product Name: MTF-1

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 454-753

Epitope Target: MTF-1

Epitope Species: human

Product Reactivity/Detection: MTF-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for MTF-1 Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent