Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MTAP CRISPR Activation Plasmid (m2): sc-426257-ACT-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MTAP CRISPR Activation Plasmid (m2) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- MTAP CRISPR Activation Plasmid (m2) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by MTAP CRISPR Activation Plasmid (m2) and MTAP CRISPR Activation Plasmid (m22) target distinct regulatory regions upstream of the Mtap transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MTAP Antibody (42-T): sc-100782
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MTAP CRISPR Activation Plasmid (m2) | sc-426257-ACT-2 | 20 µg | $397.00 |
Mouse Mtap encodes methylthioadenosine phosphorylase (MTAP), a key enzyme of the methionine salvage pathway that catalyzes phosphorolysis of 5′-methylthioadenosine to support adenine and methionine recycling and maintain cellular purine metabolism. MTAP activity links polyamine biosynthesis byproduct turnover to one-carbon metabolism and methylation capacity, influencing nucleotide pools, redox balance, and proliferative homeostasis. Loss or reduction of MTAP function is frequently associated with altered metabolic dependencies and genomic instability in cancer-relevant contexts, often in proximity to Cdkn2a locus perturbations. Mtap mouse gene editing tools enable mechanistic studies of metabolic rewiring, epigenetic regulation, and cell-state transitions, and support in vivo and in vitro models for dissecting pathway crosstalk in development and disease biology.
MTAP CRISPR Activation Plasmid (m2) provides a targeted, non-destructive approach to upregulating endogenous Mtap expression without altering the underlying DNA sequence.
MTAP CRISPR Activation Plasmid (m2) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Mtap locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Mtap transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous MTAP expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Mtap locus and enabling the study of MTAP-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of MTAP pathway restoration in tumor cells with silenced or reduced Mtap expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.